Question

can anyone tell me what the highlighter part means? what test?

Non-Fermentative Gram-Negative Bacteria Genera Pseudomonas I M + + Urea Pathogenicity Lact Skin/Respiratory - Vic Motility H2S + + + Sucrose ge

Answer 1

There is a test in microbiology known as the IMViC test. It consists of a series of individual tests to identify the species of a bacteria, especially coliforms. The four capital letters in the heading stand for each test that make up this IMViC test.

I - Indole test.

Indole test is used to determine the ability of a microorganism to split amino acid tryptophan to form the compound indole.

M - Methyl Red test.

This test determines whether the microorganism performs mixed acids fermentation when supplied glucose.

V - Voges-Proskauer test.complex

Organisms such as members of the Klebsiella-Enterobacter-Hafnia-Serratia group produce acetoin as the chief end product of glucose metabolism and form smaller quantities of mixed acids.

In the presence of atmospheric oxygen and 40% potassium hydroxide, acetoin is converted to diacetyl, and alpha-naphthol serves as a catalyst to bring out a red complex.

This test confirms the presence of there groups.

C - Citrate test.contamination

Used to distinguish between members of the Enterobacteriaceae family based on their metabolic by-products.

Citrate utilization can be used to distinguish between coliforms such as Klebsiella aerogenes which occur naturally in the soil and fecal coliforms such as Escherichia coli (-ve) whose presence would be indicative of fecal contamination.