Question

. Explain the factor(s) which contribute to phosphoenolpyruvate’s observed “high energy” phosphoryl group donor potential.

. Explain the factor(s) which contribute to phosphoenolpyruvate’s observed “high energy” phosphoryl group donor potential.

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Stimulation by phosphoenolpyruvate (PEP) of Ca2+ efflux from preloaded rat liver mitochondria is associated with an enhanced respiratory rate and is dependent on the Pi concentration of the extramitochondrial medium. PEP-induced Ca2+ efflux is inhibited by concentrations of NEM, mersalyl, and DTNB that also inhibit the respiration-linked transport of Pi as well as by bongkrekic acid at concentrations that also inhibits the adenine nucleotide translocase. Since PEP can gain access to the mitochondrial matrix by means of a carrier mediated exchange with intramitochondrial adenine nucleotides, we conclude that the ability of PEP to induce Ca2+ efflux could be associated with adenine nucleotide efflux as well as Pi influx and that the stability of the accumulated Ca2+ is likely to be controlled by the intramitochondrial ATP/Pi ratio.

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. Explain the factor(s) which contribute to phosphoenolpyruvate’s observed “high energy” phosphoryl group donor potential.
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