Question

Use Table 1 and Table 2 to create (1) a graph showing alkaline phosphatase activity at different pH values and (2) a graph showing enzyme activity at different temperatures. Label the x and y axes. Be sure to use appropriate units for specific activity (from answer 3 above).

Table 1. Bacterial alkaline phosphatase activity was measured at 70�C and various pH values.

pH value	Specific activity
7.0	0.060
8.0	0.075
8.5	0.170
9.0	0.270
9.5	0.325
10.0	0.280

Table 2. Bacterial alkaline phosphatase activity was measured at pH 9.5 and various temperatures.

Temperature (�C)	Specific activity
30	0.049
40	0.101
50	0.130
60	0.205
65	0.244
70	0.276
75	0.276
80	0.325
85	0.234
90	0.211
95	0.156
100	0.081

Answer 1

The international unit for enzymatic specific activity is given by enzymatic activity of converstion of a substrate into products enzyme per unit substrate per unit time and is given by μmol min⁻¹mg⁻¹

The graphs between enzyme activity and pH and that between enzyme activity and temperature can be found as given below:

0.35 0.3 0.25 20.2 드 0.15 0.1 0.05 0 やゆ 30 40 50 60 65 70 75 80 85 90 95 100 Temperature (°C)