Question

Lab Exercise 14 4. Lane 4, the undigested plasmid, may contain more than one band. Discuss some possi reasons why there would be more than one hand if no restriction enzymes were used 5. Why do you think the migration distance and size estimates are different for fragments 1 and 2 compared to the supercoiled DNA in lane 4? Consider the factors that affect DNA migration, as well as the conformation of the DNA (i.c., linear or circular). Based on this comparison, is PAMP circular or linear? Explain your answer. 6. Given your answers to the above questions, what do the fragments in lane 3 tell you 7. Explain how the fragments in lane 3 were formed. 8. What was the purpose of tube 4? Of tube 5? actical applications for the use of restriction endonuclease? 9. What are some practical applications for the use of restrictio

Answer 1

A4. Plasmids without restriction enzymes contain multiple bands because in the cells plasmids are present in different conformations; supercoiled, coiled, circular, linear. Therefore, each confirmation of the same plasmid represents a different band.

For the rest of the question the figures are required in which the lanes are shown.