Briefly explain how 2D-gel electrophoresis works and specify what types of molecules it detects.
Briefly explain how 2D-gel electrophoresis works and specify what types of molecules it detects.
Homework Answers
Ans. 2-D gel electrophoresis can be explained as the process in which the sample under detection is placed in the gel having a pH gradient. As the electric field starts, the proteins start moving along the pH gradient, untill it lost its charge. This location of the protein in the gel, where it has stopped marks the apparnt pH of that protein.
IEF or Isoelectric focussing is an important step in the 2-D gel electrophoresis. It refers to solubilizing the proteins without charged detergents. Generally, for this purpose, high concentrated urea solution, chaotrophs and reducing agents are used. A chaotroph is a molecule in a solution that disrupt the hydrogen bonding in the water molecules or present in the protein structure.
Mostly IEF is performe along with the immobilized pH gradient gels (IPG gels)
2-D gel electrophoresis can detect complex proteins from the different cells, tissues and from certain diagnostic biological samples too.
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Briefly explain how 2D-gel electrophoresis works and specify
what types of molecules it detects.
IN YOUROWN WORDS, briefly explain how gel electrophoresis works.
IN YOUROWN WORDS, briefly explain how gel electrophoresis works.
Exercise 14) DNA Profiling Understand the purpose gel electrophoresis and how it works. Exercise 15) Which biologic...
Exercise 14) DNA Profiling Understand the purpose gel electrophoresis and how it works. Exercise 15) Which biological molecule did we extract from peas?
Exercise 14) DNA Profiling Understand the purpose gel electrophoresis and how it works. Exercise 15) Which biological molecule did we extract from peas?
How does vertical gel electrophoresis differ from horizontal gel electrophoresis? What is the purpose of each?...
How does vertical gel electrophoresis differ from horizontal gel electrophoresis? What is the purpose of each? Are the outcomes the same?
What is the purpose of gel electrophoresis? How is size related to movement through a gel?...
What is the purpose of gel electrophoresis? How is size related to movement through a gel? What is a DNA ladder? Why is it important in gel electrophoresis and how is it used? (note: a ladder is also called a “standard”) What are two indications one can look for to be certain the gel electrophoresis is occurring? What are two strategies to improve the resolution of DNA bands?
Refer to the picture included in question 7 for this question. Please explain how gel electrophoresis...
Refer to the picture included in question 7 for this question.
Please explain how gel electrophoresis separates the DNA fragments
that are produced by the Sanger technique. Include the following
elements within your explanation (not necessarily in order of how
you should place them in your answer):
electric current and charge
size of DNA molecules
DNA samples
gel
wells
and buffer solution.
I I III I
Gel electrophoresis. Polypeptides can be separated by electrophoresis according to their relative content of acidic and...
Gel electrophoresis. Polypeptides can be separated by electrophoresis according to their relative content of acidic and basic residues. The isoelectric point (pI) of a polypeptide is the pH at which its net charge is zero. (See Fig 3.11 of your textbook). A) At physiological pH (= 7.4), what is the net charge on the tripeptide Asp-Arg-Glu-His? B) Calculate the pI of this tetrapeptide. [Hint: Using the pK, values in Table 2.1 and the Henderson-Hasselbalch equation, determine the pH when the...
6. Gel Electrophoresis separates different molecules on the basis of A) Size B) charge C) solubility...
6. Gel Electrophoresis separates different molecules on the basis of A) Size B) charge C) solubility D) both size and charge 7. Distinguish between leading and lagging strand. 8. How is DNA replication different in eukaryotes from bacteria? 9. Explain semiconservative model of DNA replication 10. Distinguish between helicases and topoisomerases
3. You observe the following while performing gel electrophoresis on two molecules at the same time...
3. You observe the following while performing gel electrophoresis on two molecules at the same time and under the same conditions. Although the molecules are the same size, you observe that molecule A barely moves from the well while molecule B moves 2 cm towards the positive electrode. Give at least one explanation to explain why these two molecules of the same size behave differently in your experiment. Molecule A - Electrode Molecule B +Electrode wells
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab activity? ___________________________________________________ __________________________________ What are the mobile phase and stationary phase substances/Compounds, which are used in this experiment? Briefly explain the purpose of each phase. ____________________________ ______________________________________ If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules pass through the opening in the bottom of the column? Mixture containing: Hemoglobin, 65,000 Daltons; Ribonuclease...
Exercise 14) DNA Profiling Understand the purpose gel electrophoresis and how it works. Exercise 15) Which biological molecule did we extract from peas?
Exercise 14) DNA Profiling Understand the purpose gel electrophoresis and how it works. Exercise 15) Which biological molecule did we extract from peas?
Refer to the picture included in question 7 for this question.
Please explain how gel electrophoresis separates the DNA fragments
that are produced by the Sanger technique. Include the following
elements within your explanation (not necessarily in order of how
you should place them in your answer):
electric current and charge
size of DNA molecules
DNA samples
gel
wells
and buffer solution.
I I III I
Gel electrophoresis. Polypeptides can be separated by electrophoresis according to their relative content of acidic and basic residues. The isoelectric point (pI) of a polypeptide is the pH at which its net charge is zero. (See Fig 3.11 of your textbook). A) At physiological pH (= 7.4), what is the net charge on the tripeptide Asp-Arg-Glu-His? B) Calculate the pI of this tetrapeptide. [Hint: Using the pK, values in Table 2.1 and the Henderson-Hasselbalch equation, determine the pH when the...
6. Gel Electrophoresis separates different molecules on the basis of A) Size B) charge C) solubility D) both size and charge 7. Distinguish between leading and lagging strand. 8. How is DNA replication different in eukaryotes from bacteria? 9. Explain semiconservative model of DNA replication 10. Distinguish between helicases and topoisomerases
3. You observe the following while performing gel electrophoresis on two molecules at the same time and under the same conditions. Although the molecules are the same size, you observe that molecule A barely moves from the well while molecule B moves 2 cm towards the positive electrode. Give at least one explanation to explain why these two molecules of the same size behave differently in your experiment. Molecule A - Electrode Molecule B +Electrode wells
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab activity? ___________________________________________________ __________________________________ What are the mobile phase and stationary phase substances/Compounds, which are used in this experiment? Briefly explain the purpose of each phase. ____________________________ ______________________________________ If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules pass through the opening in the bottom of the column? Mixture containing: Hemoglobin, 65,000 Daltons; Ribonuclease...
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