Homework Answers
Gel electrophoresis is used to separate macromolecules like DNA RNA and protiens .DNA fragments are separated according to their size.protiens can be separated according to their size and their charge , different protiens have different charges.the molecules will travel through the gel in different directions or at different speeds ,allowing them to be separated Frome one another
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Exercise 14) DNA Profiling Understand the purpose gel electrophoresis and how it works. Exercise 15) Which biologic...
What is the purpose of gel electrophoresis? How is size related to movement through a gel?...
What is the purpose of gel electrophoresis? How is size related to movement through a gel? What is a DNA ladder? Why is it important in gel electrophoresis and how is it used? (note: a ladder is also called a “standard”) What are two indications one can look for to be certain the gel electrophoresis is occurring? What are two strategies to improve the resolution of DNA bands?
How does vertical gel electrophoresis differ from horizontal gel electrophoresis? What is the purpose of each?...
How does vertical gel electrophoresis differ from horizontal gel electrophoresis? What is the purpose of each? Are the outcomes the same?
Stuck answering the rest of these 3. Application of DNA gel electrophoresis. DNA gel electrophoresis is...
Stuck answering the rest of
these
3. Application of DNA gel electrophoresis. DNA gel electrophoresis is commonly used in determining familial relationships among individuals, for ex; to establish paternity of a child. This technique is called DNA fingerprinting. In this technique the DNA of parents and children is roughly chopped up into pieces and resolved on an agarose gel. The DNA ill resolve according to their sizes and create a pattern or a "fingerprint". The fingerprint of the child is...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively charged (with a charge of two electrons per base pair). When you “run the gel” you are generating an electric field by connecting anodes and cathodes at the ends of the gel. This causes the negatively charged DNA segments to move towards the positive electrode. After running the gel, smaller DNA segments have moved farther from the...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
SHORT ANSWER 1. Explain how the technique of DNA fingerprinting works. 2. What is the purpose...
SHORT ANSWER 1. Explain how the technique of DNA fingerprinting works. 2. What is the purpose of using a restriction enzymes? 3. What is the technique of gel electrophoresis used for? 4. What is the overall charge on DNA and what gives DNA this charge? 5. If both the parents are carriers of sickle cell disease, what is the probability of having a child that has sickle cell disease? Draw a punnett square to determine the probabilty.
14. Like a ladder. Circular DNA from the SV40 virus was isolated and subjected to gel...
14. Like a ladder. Circular DNA from the SV40 virus was isolated and subjected to gel electrophoresis. The results are shown in lane A (the control) of the adjoining gel patterns. (a) Why does the DNA separate in agarose gel electrophoresis? How does the DNA in each band differ? (b) What types of DNA do the various bands represent? (c) What is the significance of the fact that more of the DNA is in slower-moving forms?
To understand how electrophoresis might work we will start with a simple model. We will ignore...
To understand how electrophoresis might work we will start with a simple model. We will ignore the gel and assume that the only force acting on the DNA segments is the electric force due to a constant electric field. Starting with a free body diagram, produce an equation that describes how the electrostatic force acts on DNA segments of different sizes. Use Newton’s 2nd law to decide how this force would affect the motion of different-sized segments of DNA. Does...
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut...
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut with restriction enzynes. A. 5 '-ACTGACATAGGCACCCCTTAA-3 3'-TGACTGTATCCGTGGGG-5 5 '-TGACTGTATCCGTGGGG-3' 3 '-ACTGACATAGGCACCCCTTAA-5' 5 '-GGCATACTAGATCCACGTTAA-3 3'-CCGTATGATCTAGGTGC-5 5 '-GGCATACTAGATCCACGAATT-3 3'-CCGTATGATCTAGGTGC-5 E. 5 '-GGCATACTAGATCCACGGATC-3 3'-CCGTATGATCTAGGTGC-5 a. Which pair of these fragments has appropriate complementary sticky ends to get joined together in a recombinant DNA molecule? b. What enzyme would we use to join up the DNA backbones to make the make the recombinant molecule?
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making...
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
Stuck answering the rest of
these
3. Application of DNA gel electrophoresis. DNA gel electrophoresis is commonly used in determining familial relationships among individuals, for ex; to establish paternity of a child. This technique is called DNA fingerprinting. In this technique the DNA of parents and children is roughly chopped up into pieces and resolved on an agarose gel. The DNA ill resolve according to their sizes and create a pattern or a "fingerprint". The fingerprint of the child is...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
SHORT ANSWER 1. Explain how the technique of DNA fingerprinting works. 2. What is the purpose of using a restriction enzymes? 3. What is the technique of gel electrophoresis used for? 4. What is the overall charge on DNA and what gives DNA this charge? 5. If both the parents are carriers of sickle cell disease, what is the probability of having a child that has sickle cell disease? Draw a punnett square to determine the probabilty.
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut with restriction enzynes. A. 5 '-ACTGACATAGGCACCCCTTAA-3 3'-TGACTGTATCCGTGGGG-5 5 '-TGACTGTATCCGTGGGG-3' 3 '-ACTGACATAGGCACCCCTTAA-5' 5 '-GGCATACTAGATCCACGTTAA-3 3'-CCGTATGATCTAGGTGC-5 5 '-GGCATACTAGATCCACGAATT-3 3'-CCGTATGATCTAGGTGC-5 E. 5 '-GGCATACTAGATCCACGGATC-3 3'-CCGTATGATCTAGGTGC-5 a. Which pair of these fragments has appropriate complementary sticky ends to get joined together in a recombinant DNA molecule? b. What enzyme would we use to join up the DNA backbones to make the make the recombinant molecule?
Gel Electrophoresis and Bioinformatics Analyses of wild type and mutant Arabidopsis 1. The procedure for making 30 mL of 1.2% agarose in 1x TAE is: g agarose mL lX TAE 2. Briefly explain the roles of each of the following in electrophoresis. Agarose - Tris - EDTA- Power supply - 3. What is the stain we used to view DNA and how does it work? 4. Which samples amplified and which ones did not?
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