Although lipid-linked proteins and peripheral membrane proteins both exist outside of the plane of the membrane,...
Although lipid-linked proteins and peripheral membrane proteins both exist outside of the plane of the membrane, one can be removed somewhat easily whereas removing the other requires disrupting the membrane. Which type of protein requires the membrane to be disrupted to isolate it? Explain your choice. (Describe the connection each protein has to the cell membrane and explain why the membrane must be disrupted to isolate one of the proteins.)
Homework Answers
Lipid-anchored proteins are located on the cell membrane. They are covalently attached to the membrane lipids. Ex: GPI-anchored proteins
Peripheral proteins are also located on the cell membrane. They are associated with membrane via hydrophobic interactions. Ex: Cytochrome c
We do not have to disrupt the membrane to remove peripheral proteins. On the other hand, the membrane has to be disrupted to remove anchored proteins.
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Although lipid-linked proteins and peripheral membrane proteins
both exist outside of the plane of the membrane,...
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How many weeks, days,& hours would it take to see a
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quality in the airline industry?
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there to extend auch programmes to external partners?
3. What challenges may BA face in implementing an internal
marketing programme to deliver value to its customers?
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Match the following: Integral (intrinsic) membrane proteins Transmembrane protein Porins C-- Lipid-linked proteins Peripheral (extrinsic) proteins 1. Channel-forming proteins found in the outer membranes of bacteria, with a beta-barrel motif. 2. Proteins that are associated with membranes, but can be dissociated by relatively mild procedures. 3. Proteins that completely span the membrane. 4. A general class of proteins that are tightly bound to membranes by hydrophobic interactions. 5. Membrane-associated proteins that have covalently-bonded lipids.
SDS-PAGE is often used to determine the location and orientation of membrane associated proteins. Commonly, experimental preparations of membranes are treated in a variety of ways in order to extract the proteins, which are then subjected to SDS-PAGE. These treatments will affect membrane proteins in different ways, based on how they are arranged in the membrane. One of the most common pre-treatments is by the digestive enzyme trypsin, which degrades polypeptide chains. Trypsin is too large to cross a membrane,...
Question 24 Which of the following categories of membrane proteins is most likely to have restricted lateral mobility in a membrane? enzyme transporter O anchor O receptor all are restricted Question 5 New membrane synthesis occurs by spontaneous aggregation of free phospholipids into a new bilayer incorporation of phospholipids into one face of a preexisting membrane and their specific redistribution by enzymes like flippases incorporation of phospholipids into both faces of a preexisting membrane at the same time formation of...
A membrane consisting only of phospholipids undergoes a sharp transition from the crystalline form to the fluid form as it is heated. However, a membrane containing and 80% phospholipid and 20% cholesterol undergoes a more gradual change from crystalline to fluid form when heated over the same temperature range. Explain why. Purification of transmembrane proteins requires the addition of detergents to the buffets in order to solubilize the proteins. (a) Why would transmembrane proteins be insoluble if the detergent were...
BACKGROUND:
Mycobacterium tuberculosis causes tuberculosis, one of the world’s
deadliest diseases. It is an acid-fast bacillus that is 2-4 um in
length and 0.2-0.5 um in width.
How many weeks, days,& hours would it take to see a
M.tuberculosis colony on solid media? Its generation time is listed
in the reading (above). Assume that bacterial colonies are not
visible to the naked eye unless they contain ≥ 2 millioncells. This
species forms aggregates of 5 cells on average. Show your...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
Discussion questions
1. What is the link between internal marketing and service
quality in the airline industry?
2. What internal marketing programmes could British Airways
put into place to avoid further internal unrest? What potential is
there to extend auch programmes to external partners?
3. What challenges may BA face in implementing an internal
marketing programme to deliver value to its customers?
(1981)ǐn the context ofbank marketing ths theme has bon pururd by other, nashri oriented towards the identification of...
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