Homework Answers
Answer B- forms a dimer, binds to two distinct site and forms a DNA loop that blocks access of RNA polymerase to arabinose operon promoter.
The AraC gene functions as transcription activator in presence of arabinose. In the absence of arabinose however it halts the process of transcription as the cells do no require araBAD gene and AraC gene forms a dimer and act as a repressor. Out of this dimer, one monomer binds to the araBAD gene operator and another monomer binds to the aral1 gene which is also called as the ditant DNA half site. These dimers leads to the formation of a DNA loop. This DNA loop thus formed blocks the binding of RNA polymerase to the araBAD promoter which inturn haults the process of transcription. Therefore no transcription process takes place in the absence of arabinose.
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Question 11
Question 11 ???????? In the absence of arabinose, the regulatory protein AraC a forms...
For regulation of the trp operon, what happens to in the absence of tryptophan? repressor protein...
For regulation of the trp operon, what happens to in the absence of tryptophan? repressor protein (a) It binds to the operator, and represses transcription of genes in the trp operon (b) It cannot bind to the operator, and transcription of genes in the trp operon takes place (c) It binds to the regulatory gene that codes for the TrpR repressor, and represses its transcription (d) It cannot bind to the regulatory gene that codes for the TrpR repressor, and...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
Question 13 (1 point) Alternative sigma factors initiate transcription in different genes by recognizing characteristic sequences...
Question 13 (1 point) Alternative sigma factors initiate transcription in different genes by recognizing characteristic sequences in their 1) RNA polymerase 2) activation sites 3) promoters 4) enhancer 5) operator FUSION1 u point) Genes whose transcription is controlled by the same regulatory elements are said be under: 1) positive control 2) negative control 3) allosteric control 4) coordinated control 5) epigenetic control Question 16 (1 point) In the presence of both arabinose and glucose the arabinose operon is:
Question 1 Match the term with the best definition or description; most topics relate to the...
Question 1 Match the term with the best definition or description; most topics relate to the regulation of gene expression. General type of protein which will increase transcription rates when it attaches to a site A. Factor connected to a particular gene - B. Co-repressor C. Enhancer D. Promoter E. Structural F. Intron G. Activator H. Operator I. Basal transcription J. Glucocorticoid receptor K. Sigma factor L. Mediator M. Inducer N. TATA box O. Repressor The rates of mRNA produced...
Match each term associated with genes and control of gene expression with the appropriate description. A...
Match each term associated with genes and control of gene expression with the appropriate description. A transcriptional unit" that consists of promoter multiple genes under the control of a single regulatory element. A transcriptional regulatory protein (prokaryotic or eukaryotic) which works by turning on or increasing gene transcription. activator The region of a gene to which RNA polymerase binds. Enhancer A transcriptional regulatory protein prokaryotic or eukaryotic) which works by turning off or decreasing gene transcription. repressor A molecule that...
The gene machine program shows you what happens when lactose is present in E. coli, and...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
The phoNY operon is located on a part of the bacterial chromosome in between genes C...
The phoNY operon is located on a part of the bacterial chromosome in between genes C and D. The DNA looks like: ...A B C -----D E F..... Click 5 Watson The dashed lines represent the structural genes (enzyme encoding) of the phone operon. Mutations that affect the regulation of the phoNY operon have been identified and mapped. Some of the regulatory mutations map near gene D and some map near gene A(asterisks mark approximate locations). No phoNY regulatory mutations...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
A) An Operon is:? 1- A gene that affect another gene transcription. 2. A gene coding...
A) An Operon is:? 1- A gene that affect another gene transcription. 2. A gene coding for a metabolic pathway. 3. A protein that blockgene expression. 4. A set of genes under the control of one promoter. B )- In bacteria the Lac Operon genes code for proteins that break down.? 1- Arabinose 2- Lactose 3- Galactose 4- Glucose C )mRNA processing involves removal of .......? 1- Introns 2- The poly-A tail 3- The 5' cap 4- Exons D )...
26. The lac operon in E. coli consists of genes that code for enzymes necessary for...
26. The lac operon in E. coli consists of genes that code for enzymes necessary for the breakdown of lactose. When lactose is absent, the operon is inactive because a repressor protein binds to a specific site in the lac operon. When lactose is present, lactose molecules bind to the repressor protein, causing the repressor protein to dissociate from the binding site. In the absence of glucose (a preferred energy source for bacteria), the protein CAP binds to a regulatory...
For regulation of the trp operon, what happens to in the absence of tryptophan? repressor protein (a) It binds to the operator, and represses transcription of genes in the trp operon (b) It cannot bind to the operator, and transcription of genes in the trp operon takes place (c) It binds to the regulatory gene that codes for the TrpR repressor, and represses its transcription (d) It cannot bind to the regulatory gene that codes for the TrpR repressor, and...
Question 13 (1 point) Alternative sigma factors initiate transcription in different genes by recognizing characteristic sequences in their 1) RNA polymerase 2) activation sites 3) promoters 4) enhancer 5) operator FUSION1 u point) Genes whose transcription is controlled by the same regulatory elements are said be under: 1) positive control 2) negative control 3) allosteric control 4) coordinated control 5) epigenetic control Question 16 (1 point) In the presence of both arabinose and glucose the arabinose operon is:
Question 1 Match the term with the best definition or description; most topics relate to the regulation of gene expression. General type of protein which will increase transcription rates when it attaches to a site A. Factor connected to a particular gene - B. Co-repressor C. Enhancer D. Promoter E. Structural F. Intron G. Activator H. Operator I. Basal transcription J. Glucocorticoid receptor K. Sigma factor L. Mediator M. Inducer N. TATA box O. Repressor The rates of mRNA produced...
Match each term associated with genes and control of gene expression with the appropriate description. A transcriptional unit" that consists of promoter multiple genes under the control of a single regulatory element. A transcriptional regulatory protein (prokaryotic or eukaryotic) which works by turning on or increasing gene transcription. activator The region of a gene to which RNA polymerase binds. Enhancer A transcriptional regulatory protein prokaryotic or eukaryotic) which works by turning off or decreasing gene transcription. repressor A molecule that...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
The phoNY operon is located on a part of the bacterial chromosome in between genes C and D. The DNA looks like: ...A B C -----D E F..... Click 5 Watson The dashed lines represent the structural genes (enzyme encoding) of the phone operon. Mutations that affect the regulation of the phoNY operon have been identified and mapped. Some of the regulatory mutations map near gene D and some map near gene A(asterisks mark approximate locations). No phoNY regulatory mutations...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
26. The lac operon in E. coli consists of genes that code for enzymes necessary for the breakdown of lactose. When lactose is absent, the operon is inactive because a repressor protein binds to a specific site in the lac operon. When lactose is present, lactose molecules bind to the repressor protein, causing the repressor protein to dissociate from the binding site. In the absence of glucose (a preferred energy source for bacteria), the protein CAP binds to a regulatory...
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