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Exp normal inhib1 inhib2 [S](mM) VV V(all units are umol/(mLsec)) 2 24 Assuming [Etotal] is the same in all experiments. [ 5

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Mv.poss-o-3근 =-는을s=o-oes. 6.0.062 T는.b2Sx︺ 3125-55-64 100 x 5 833

0.045 0.04 0.035 km/vmax 0.025 0.02 0.015 0.01 0.005 0.1 0.3 0.2 0.5 0.6

0.045 Inhibitor 1 mpetitive inhibitor 0.04 0.025 0.02 0.015 -se ne sl Linear (Series1) 005 0.6 - 1/Km appE-0.18 Km app 5.55 m

0.045 0.04 0.035 Inhibitor 2 Un competitive 1/Vmaxapp 0.025 Vmaxapp 40 0.025 0.02 0.015 0.01 0.005 -Series1 0 0.1 0.2 3 0.4 0

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