Question

Microbiology - Clinical Microbiology and Rapid Testing

[Q1]

You have just completed an API 20E on an unknown sample. Interpret the results shown in the incubated strip and oxidase test below. Assume that all the finishing reagents have been correctly added.

API 20E Interpretation Key Test ONPG Negative Result Colorless ADH Yellow LDC Yellow Positive Result Yellow Red/Orange Red/Orange Red/Orange Blue-green/blue Black deposit forms Red/Orange Reddish brown ODC CIT H2S Yellow Pale green/yellow Colorless URE Yellow TDA Yellow IND Pink Colorless/yellow Colorless VP Pink/red

[Q2]

Determine the scores and profile number for the strip result table shown below.

Answer 1

1.

Test

ONPG

ADH

LDC

ODC

CIT

H2S

URE

TDA

IND

VP

GEL

GLU

Color

Colorless

Orange

Yellow

Red

Blue-green

Black

Red

Brown

Lt yellow

Colorless

Black

Yellow-green

Result

-

+

-

+

+

+

+

+

-

-

+

+

Test	MAN	INO	SOR	RHA	SEC	MEL	AMY	ARA	OX
Color	Blue	Blue	Blue	Blue	Blue	Blue	Blue	Blue	No change
Result	-	-	-	-	-	-	-	-	-