Question

2. In DNA replication, the ________ strand grows towards the replication fork, while the ________ strand grows away from
   the replication fork.

1. mRNA; leading
2. leading; lagging
3. leading; template
4. lagging; template
5. lagging; leading

3. Which of the following is not necessarily related to tumour formation?

1. an overactive MYC gene
2. proto-oncogenes
3. inactive tumour-suppressor genes
4. cell de-differentiation
5. metastasis

4. The total number of unique, three-base combinations of the four nucleic acid bases in DNA is

1. 12.
2. 16.
3. 20.
4. 64.
5. 256.

5. The purpose of cloning is

1. to sequence a particular gene.
2. to obtain large numbers of a particular gene.
3. to obtain plasmids with a variety of genes.
4. to insert genes into plasmids
5. all of the abov

6. Which of the following is NOT correct for PCR?

1. It produces large amounts of DNA in a host, usually a bacterium.
2. It requires the presence of primers.
3. It can produce DNA from the root of a single human hair.
4. Some steps are carried out at high temperatures.
5. It works with a heat resistant DNA polymeras

Answer 1

1. Option B, leading strand moves in 3' to 5' direction towrds fork while lagging moves 5'to3' direction away from fork.

2. Option A, an overactive MYC gene, this gene results in gene expression not division so it do not induce tumor formation.

3. Option D, 64, this is a triplet code

4. All of above

5. Option A, It produces large amounts of DNA in a host, usually a bacterium.

PCR is a machine in which amplification of dna takes place not in bacteria

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