Question

Vocabulary: DNA Replication 

A. Helicase B. Primase C. Single Strand Binding Protein (SSB) D. Topoisomerase E. Origin of Replication F. DNA Polymerase G. Leading Strand H. Lagging strand I. DNA Ligase J. Okazaki Fragment K. Replication Fork L. RNA Primer M. Topoisomerase 

.1. Site where the replication of a DNA molecule begins. 

2. The new continuous complementary DNA strand synthesized in the direction for the replication fork. 

3. A discontinuously synthesized DNA strand that elongates in a direction away from the replication fork 

4. Relaxes DNA twisting ahead of the replication fork as the helicase moves down the DNA 

5. A short stretch of RNA bound to template DNA to which DNA nucleotides are added during DNA synthesis 

6. Relaxes supercoiling ahead of the replication fork 

7. Enzymes that catalyze elongation of a new DNA strand during replication on the leading strand and short segments on the lagging strand 

8. An enzyme that joins RNA nucleotides to make a primer 

9. An enzyme that joins (ligates) the sugar-phosphate backbone of the Okazaki fragments to create a single DNA strand. 

10. Proteins that line up along the unpaired DNA strands during replication to hold them apart 

11. Short fragment of DNA synthesized on the lagging strand away from the replication fork. 

12. A Y-shaped region on a replicating DNA molecule where new strands are forming.

Answer 1

DNA Replication Vocabulary :-

1. (E) Origin of replication
2. (G) Leading strand.
3. (H) Lagging strand
4. (D) Topoisomerase
5. (L) RNA primer.
6. (M) Topoisomerase
7. (F) DNA polymerase
8. (B) Primase
9. (I) DNA Ligase
10. (C) Single strand binding protein (SSB)
11. (J) Okazaki fragment
12. (K) Replication Fork.

• Topoisomerase vs helicase enzyme :-
Helicase unwinds the double-stranded DNA whereas topoisomerase relieves the tension created by helicase enzyme.

• Leading strand is replicated continuously whereas lagging is replicated discontinuously.

Replication process summary :-

SSBP: SINGLE STRAND BINDING PROTEIN PRIMASE DNA POLYMERASE SSBP ha LEADING STRAND ADDED NUCLEOTIDES ANA PRIMER TTT MAT DNA HELI CASE TAL OKAZAKI FRAGMENTS DNA GYRA SE 10 LAGG2116 STRAND sus LIGASE

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