Question

A B C D E F G H I gene - estrogen + estrogen (fig74) Gene expressed higher in tumor vs normal cells Gene expressed lower in tumor vs normal cells Gene expressed the same in tumor vs normal cells Gene expressed neither in tumor nor normal cells The figure above shows a portion of the results from an experiment comparing the mRNA levels determined by RNAseq in cells extracted from an ovarian tumor to normal ovary cells. Both groups of cells were also treated with estrogen or no estrogen (a hormone produced by the ovary) before RNA isolation and RNAseq. Shown are the relative RNA levels of 9 genes (A-1) in the different conditions. Based upon the comparison of RNA levels between tumor and normal cells: A) Which gene(s) could potentially be proto-oncogenes? Explain. (2 pts) B) Which gene(s) could potentially be tumor suppressor genes? Explain. (2 pts) C) Which gene(s) are being activated in the tumor in response to estrogen? (1 pt)

Answer 1

1) Genes A and B .

       Because they are active in either case when estrogen is present and absent.

2)Genes E and F.

      Tumor suppressor genes are active in normal cells but lessactive/inactive in cancers cell.

3) Gene I is activated in the tumor in response to estrogen.