Question

estion 18 and Two-dimensional gel electrophoresis separates proteins based on a. size; shape b.shape; charge C. size; concentration d. size; charge e concentration; shape

Answer 1

Answer:

18.(d) size,Charge

In two dimension gel electrophoresis of isolated proteins, first they are subjected to Isoelectric isolation and then size exclusion. As different proteins has different amino acid composition, they have different charges on them. So they are first separated on the basis of their charge . Then they are treated with SDS so that they are all negatively charged, PAGE is done in a vertical manner to the previous process. PAGE helps in separation of proteins in the basis of size.

14.(c) the percentage of the genome devoted to coding sequence in eukaryotic genome is lower than in prokaryotic genome.

Prokaryotes are simple and primitive organisms but they use their genome rather efficiently. About 88% of their genome is coding genome. Whereas in eukaryotes like human, only 2% of the genome is coding portion. There is no certain explanation for this fact. Eukaryotes have multiple origin and prokaryotes have single origin. Being more sophisticated, it has more regulatory sequences and is larger than prokaryotic genome. Both eukaryotes and prokaryotes have transposons. So all these facts are wrong in the options and only (c) is the correct answer.

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