Question

Normal No Spacing Heading 1 hmi Biointeractive Using CRISPR to identify the Functions of Butterfly Genes Activity Student Handout Figure 2. V. cerea butterflies (lett: wild-type control; right: getic knockout). Figure 3. Wings of A voules butterflies (left: wild-type control right: optix knockout). Figure 4. Wings of J. sesaug, butterflies left; wild-type control;right: esta knockout). a. For each species shown, describe how the wings of the entix knockout compare to those of the wild- type butterfly b. Based on the figures, predict the function of the entir.gene. Does the gene have the same function in all three butterfly species? If not, explain how it may differ among the species. c. Justify your prediction with evidence from all three figures. Be specific 6. Launch the CRISPR-Cas9: Mechanism & Applications Click & Learn and select "How It's Used." Scroll through the videos on the right side of the screen and watch the three video interviews with Robert Reed. Revise your answers to Question 6 based on information from the videos.

Answer 1

1. For the optic knockout, in species V. cardui butterfly, the orange color is not present in the wings but the pattern is the same; in the species A. vanillae, the orange color is absent but the pattern is consistent and same can be said for the J. coenia but here the blue pattern is observed in the knockout but the pattern is the same.

2. The function of the optic gene is to produce color in the wings in all the three species but in the last species, the background blue color when optix gene is knocked out is seen.

3. As seen in figure 2 and 3, the orange color of the wings are lost but the pattern on the ring is the same and the pattern is the same in the figure 4 but the color is different. But overall the optix gene is a color coding or color determining gene.

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