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9. Blood types are determined by A. Agglutination B. Precipitation C. Ouchterlony

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Answer: A. Agglutination.

Agglutination process is used for determining the blood group. It is an antigen-antibody reaction and it is the process in which antigens bind with its specific antibodies forming visible clumps. During the agglutination reaction, the insoluble RBC antigens binds with the soluble antibodies (Anti-A or Anti-B) and forms visible clumps. For the blood typing, a drop of blood is added on to slide coated with Anti-A on one end and Anti-B antibody on the other end. The person having blood group A will have anti-B antibodies in the blood, hence, agglutination will take place on the  Anti-B coated side of the slide. If the blood group is B, then the agglutination will takes place on the Anti-A coated side. If no agglutination reaction takes place, the blood group is AB. Agglutination reaction with both Anti-A and Anti-B is not present, then the blood group is 'O'.

Precipitation is a process is the process in which the ions present in the solution reacts leading to the formation of insoluble clumps. Precipitation process occurs for the soluble antigen and antibodies. The RBC antigens are insoluble in nature.

Ouchterlony test is a type of immuno diffusion used for the detection and quantification of antigens and antibodies present.

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