25. Which is not a required reactant for a PCR reaction? Select one: a. Primer DNA...
25. Which is not a required reactant for a PCR reaction?
Select one:
a. Primer DNA
b. Taq polymerase
c. dNTP
d. template DNA
e. ATP
Homework Answers
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25. Which is not a required reactant for a PCR reaction?
Select one:
a. Primer DNA...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
Eliminating which of the following from a PCR reaction would result in no amplification of DNA?...
Eliminating which of the following from a PCR reaction would result in no amplification of DNA? a) template DNA b) primers c) Taq Polymerase d) dNTPs e) Eliminating any of these would result in no amplification of DNA
Some of the PCR techniques for DNA amplification are: Regular PCR Hot start PCR High-Fidelity PCR...
Some of the PCR techniques for DNA amplification are: Regular PCR Hot start PCR High-Fidelity PCR Immuno PCR. Real-time PCR ------------------------------------ 2. The following information about Taq DNA polymerase is/are correct. Taq DNA polymerase was discovered by Kary Mullis in 1983. The entire Taq DNA polymerase reaction (PCR) technique was bought by Perkin Elmer in the range of $120 million. Kary Mullis received a Nobel prize in 1993 for discovering Taq DNA polymerase. Taq DNA polymerase was isolated from Thermus aquaticus....
Which of the following molecules is not required for a PCR reaction? View Available Hint(s) Ligase...
Which of the following molecules is not required for a PCR reaction? View Available Hint(s) Ligase O Primer O DNA O DNTPs Submit Previous Answers X Incorrect; Try Again; One attempt remaining dNTPs are required because they are the building blocks of DNA molecules. - Part B The thermostability of Taq polymerase is required during the annealing phase of PCR. View Available Hint(s) O True O False Submit
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25...
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25 bases when the temperature is increased in the PCR machine from 50'C to 90C.
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25 bases when the temperature is increased in the PCR machine from 50'C to 90C.
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25...
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25 bases when the temperature is increased in the PCR machine from 50'C to 90C.
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25 bases when the temperature is increased in the PCR machine from 50'C to 90C.
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in th...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
10. For each of the following reagents briefly explain their role in a PCR reaction (3...
10. For each of the following reagents briefly explain their role in a PCR reaction (3 points each) a. dNTP b. DNA containing the sequence to be amplified. c. DNA ligase. d. Heat-stable DNA polymerase. e. Oligonucleotide primer(s).
5. Which of the following single-stranded DNA sequences is most likely to form a stem-loop structure? a. GACCGTATGC...
5. Which of the following single-stranded DNA sequences is most likely to form a stem-loop structure? a. GACCGTATGCACGGTC b. TCATAGGCGCCGTTCA c. GGATCACGTTACCGCC d. ATAAGATGGGAGCATG The structures below represent the 8 D stereoisomeric aldohexoses. Answer the following (6-7) based on this figure Si carbons ---OH H= =QH --OH H--OH CH,OH HO--- H=CHCH -- - OH CH,OH ---CH HỌC 6 -- - OH Chon D-Glucose HO-- H H -- - OH CH,OH -Marnese -- Hà QH HO--- - OH CHOH Giese HO--...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)?...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)? Question 16 options: A) Nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA B) A restriction endonuclease enzyme that cleaves DNA at specific locations C) The segment of DNA that must be copied D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
Which of the following molecules is not required for a PCR reaction? View Available Hint(s) Ligase O Primer O DNA O DNTPs Submit Previous Answers X Incorrect; Try Again; One attempt remaining dNTPs are required because they are the building blocks of DNA molecules. - Part B The thermostability of Taq polymerase is required during the annealing phase of PCR. View Available Hint(s) O True O False Submit
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25 bases when the temperature is increased in the PCR machine from 50'C to 90C.
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25 bases when the temperature is increased in the PCR machine from 50'C to 90C.
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25 bases when the temperature is increased in the PCR machine from 50'C to 90C.
Estimate the change of the diffusion value (in percentage) of a PCR primer (DNA template) with 25 bases when the temperature is increased in the PCR machine from 50'C to 90C.
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
5. Which of the following single-stranded DNA sequences is most likely to form a stem-loop structure? a. GACCGTATGCACGGTC b. TCATAGGCGCCGTTCA c. GGATCACGTTACCGCC d. ATAAGATGGGAGCATG The structures below represent the 8 D stereoisomeric aldohexoses. Answer the following (6-7) based on this figure Si carbons ---OH H= =QH --OH H--OH CH,OH HO--- H=CHCH -- - OH CH,OH ---CH HỌC 6 -- - OH Chon D-Glucose HO-- H H -- - OH CH,OH -Marnese -- Hà QH HO--- - OH CHOH Giese HO--...
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