Question

1. Some of the PCR techniques for DNA amplification are:

		Regular PCR
		Hot start PCR
		High-Fidelity PCR
		Immuno PCR.
		Real-time PCR

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2. The following information about Taq DNA polymerase is/are correct.

		Taq DNA polymerase was discovered by Kary Mullis in 1983.
		The entire Taq DNA polymerase reaction (PCR) technique was bought by Perkin Elmer in the range of $120 million.
		Kary Mullis received a Nobel prize in 1993 for discovering  Taq DNA polymerase.
		Taq DNA polymerase was isolated from Thermus aquaticus.
		It is a bacterial enzyme for DNA replication.

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3. The following are true foe the specific PCR technique:

		Tgo DNA Polymerase maintains four time the fidelity DNA amplification ib comparison to Taq DNA Polymerase.
		High Fidelity PCR uses a combination of Taq DNA Polymerase and Tgo DNA Polymerase.
		Tgo DNA Polymerase has 3’-5’ exonuclease or proofreading activity.
		Long Template PCR system uses Taq DNA Polymerase and thermostable DNA polymerase.
		The error rate is decreased 30 folds by High Fidelity PCR in comparison toTaq DNA Polymerase.

Answer 1

Question

2. The true statements about polymerase chain reaction are:

I. It is obtained from the Thermus aquaticus bacteria.

II. It is the bacterial enzyme used for DNA replication.

Hence option d, e are correct.

3. High Fidelity PCR uses the combination of Taq polymerase DNA and pfu. The high Fidelity PCR increases the rate of replication by 4 fold. Hence option b and e are correct.