Question

Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were grown with or without glucose, and with or without lactose (for example, with glucose and without lactose is indicated by +/-). Indicate (Yes or No) whether maximum transcription at the chromosomal lac operon promoter occurs in the strain indicated under the conditions given. Plasmid None Glucose/Lactose CAP Bound? Laci Bound? Maximum Transcription? - / + Yes +/- No -/- lacit None laco+ + / + +/- None +/+ Chromosome crpt laci lacot lacz+ lacrt crpt laci lacot lacz+ lacyt crpt lacit laco lacz+ lacrt crpt lacit laco laczt lacyt crplacit lacot lacz+ lacy+ crplacit lacot lacz+ lacyt crpt lacit lacot lacz lacrt crpt lacit lacot lacz lacrt crpt lacit lacot lacz lacy crpt lacit lacot lacz lacy crpt lacit lacot laczt lacy crpt lacit lacot laczt lacy Submit Answer Tries 0/99 crpt None +/- lacz+ -/+ None +/- lacz+ - / + None - / + lazyt + / -

Answer 1

Results are shown below:

When permease is non-functional,and others are wild-type ,presence of lactose and absence of glucose will not give maximum transcript because,lactose molecule is not able to enter the cell.

Chromosome	CAP bound	lac I bound	Maximum Transcriotion
1	No	No	Yes
2	Yes	Yes	No
3	Yes	No	Yes
4	No	Yes	Yes
5	No	Yes	No
6	No	Yes	No
7	No	Yes	No
8	Yes	No	Yes
9	No	Yes	No
10	Yes	Yes	No
11	Yes	Yes	No
12	No	Yes	No