Question

9. Describe the ping-pong mechanism using Lineweaver-Burk plot? (5pts)

Answer 1

The ping-pong Mechanism. : - One substrate binds first to the enzyme of ollowed by is a product Prelease ... Typically, products is fragment of the original substrate substrate is covalently attached to the designated as €. Now the second reactant, B, binds and reacts with the E and harms A. The rest of the enzyme &, melich is a the Covalent bond to the fragment of A still at attached to enzyme , forming product &. Tws is now released and the me as stored to its initial form, &. This represents a mechanism. enzyme ping-pong

and the me enzyn represents is stored to its initial form, &. This mechaniem. ping-pong A 135 15 (EAT-> EP) (EBL=760) Xo B B2 BT B3 YA

One example of a prog-pong enzyme is low molecular Line wean -weance - Burk plots character of ping-pong kinetico small substrate the enzyme covalently of the product weight protein tyrosine phosphate. It reacts with the p-in tropheryl phosphate (A) which binde to with the explusion P, the p-nitrophenol leaving group. Water (13) then (3) then comes covalently with the explusion of the products, the enzyme, forming it as inorganic phosphate. in and attacks sue an adduct with recovalently bound phosphate releasing