Question

JU DLO:05am Instructions Question 54 7.5 (0030) Why are dideoxyribonucleoside triphosphates used during DNA sequencing? They cannot be incorporated into DNA by DNA polymerase. They are incorporated into DNA particularly well by DNA polymerases from thermophilic bacteria. Incorporation of a dideoxyribonucleoside triphosphate leads to the termination of replication for that strand. Dideoxyribonucleoside triphosphates are more stable than deoxyribonucleoside triphosphates. < Previous Next Not saved Sub

Answer 1

The answer is option C

the addition of the diideoxyribonucleotide triphosphates,stops the polymerization of the DNA strand during the replication process.This helps in producing DNA strands of varying length. The DdNTP lacks a 3'-OH group on the pentose sugar structure when compared to the dNTP.Instead of that a hydrogen group is present on the 3' position.Theabsence of the OH group results in the termination of DNA polymerisationas the polymerization needs a 3'-OH group to continue.