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Question 7 The two conditions for negative stain are NO STRONG CHEMICAL: NO NUTRITION ONO HEAT FIX: NO MULTIPLICATION NO STRO
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Negative staining technique is a method that is used for diagnostic purposes to view the contrasting images of specimen. The staining method is direct and in this case the organism is not stained and kept untouched but the background is stained in order to view a contrasting feature of the organism. This process does not require any heat fixing because the staining is directly applied along with the organism. Negative stains can be sometimes heavy chemicals like osmium tetraoxide for the Electron microscopy so they sometimes can be strong chemicals. But because there is no multiplication of the organisms during the stain because that is not possible.

The correct answer is : no heat fix ; no multiplication

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