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Expansion of the Universe Exercise Student Name: Step 1. 1. Using the data in Figures 1-4,...
1. Graphical Analysis (6 p). Using only the D2L EXCEL data for the UNKNOWN you are...
1. Graphical Analysis (6 p). Using only the D2L EXCEL data for the UNKNOWN you are assigned to perform a graphical analysis. Paste-in all the graphs for a complete analysis. Include the locax equation of the line and the R2 value for each. Unknown Number Lambda Max (nm) Molar Absorptivity (M^-1 cm^-1) Temperature (°C) 15 226 6.00E+04 24 y = -0.0016x + 0.9189 R? = 0.9962 Abs vs Time 1.000 0.900 0.800 0.700 0.600 0.500 0.400 0.300 0.200 0.100 0.000...
Secilon # TA: 90 Table 1: Time, absorbance, and calculated absorbance data. Time Abs In(Abs) 1/Abs...
Secilon # TA: 90 Table 1: Time, absorbance, and calculated absorbance data. Time Abs In(Abs) 1/Abs | 0 1 .380 0.322 0.725 30 1.100 0.095 0.909 60 0.863 -0.147 1.159 0.677 -0.390 1.477 120 0.516 -0.662 1.938 150 0.394 -0.931 2.538 180 0.307 - 1.181 3.257 210 0.233 -1.457 4.292 240 0.182 -1.704 5.495 270 0.146 -1.924 6.849 300 0.110 -2.207 9.091 330 0.089 -2.419 11.236 0.072 -2.631 13.889 390 0.057 -2.859 17.452 420 0.049 -3.022 20.534 450 0.042 -3.180...
I am stuck on how to find concentration for standards and unknowns. Any kind of help would be appreciated. The correct...
I am stuck on how to find concentration for standards and
unknowns. Any kind of help would be appreciated.
The corrected absorbance is = Blank - absorbance
1. Prepare the BCA working reagent. Use a graduated cylinder and add 30 ml of the BCA reagent into a 50 ml Erlenmeyer flask. Use the appropriate micropipette to add 600 uL of the CuSO4 solution into the flask and mix by swirling the flask. Record the exact mass of albumin and the...
Procedure 1. Label four 50.0 mL volumetric flasks.)! through 4 2. Using a buret, add 10.00 ml of ...
Procedure 1. Label four 50.0 mL volumetric flasks.)! through 4 2. Using a buret, add 10.00 ml of 2.00x10'MFeNO)D solution to each of the flasks 3. Using a buret, add 1.00 mL. 2.00 mL, 3.00 mL, and 4.00 mL of 2.00x 10 MKSCN 4. Fill each flask to the mark with 0.10 M HNO, solution. Stopper each flask and invert 5. Measure the absorbance of each solution at a wavelength of 447 nm (the absorbance reterred to in step 1...
1. Graphical Analysis (6 p). Using only the D2L EXCEL data for the UNKNOWN you are assigned to perform a graphical analysis. Paste-in all the graphs for a complete analysis. Include the locax equation of the line and the R2 value for each. Unknown Number Lambda Max (nm) Molar Absorptivity (M^-1 cm^-1) Temperature (°C) 15 226 6.00E+04 24 y = -0.0016x + 0.9189 R? = 0.9962 Abs vs Time 1.000 0.900 0.800 0.700 0.600 0.500 0.400 0.300 0.200 0.100 0.000...
Secilon # TA: 90 Table 1: Time, absorbance, and calculated absorbance data. Time Abs In(Abs) 1/Abs | 0 1 .380 0.322 0.725 30 1.100 0.095 0.909 60 0.863 -0.147 1.159 0.677 -0.390 1.477 120 0.516 -0.662 1.938 150 0.394 -0.931 2.538 180 0.307 - 1.181 3.257 210 0.233 -1.457 4.292 240 0.182 -1.704 5.495 270 0.146 -1.924 6.849 300 0.110 -2.207 9.091 330 0.089 -2.419 11.236 0.072 -2.631 13.889 390 0.057 -2.859 17.452 420 0.049 -3.022 20.534 450 0.042 -3.180...
I am stuck on how to find concentration for standards and
unknowns. Any kind of help would be appreciated.
The corrected absorbance is = Blank - absorbance
1. Prepare the BCA working reagent. Use a graduated cylinder and add 30 ml of the BCA reagent into a 50 ml Erlenmeyer flask. Use the appropriate micropipette to add 600 uL of the CuSO4 solution into the flask and mix by swirling the flask. Record the exact mass of albumin and the...
Procedure 1. Label four 50.0 mL volumetric flasks.)! through 4 2. Using a buret, add 10.00 ml of 2.00x10'MFeNO)D solution to each of the flasks 3. Using a buret, add 1.00 mL. 2.00 mL, 3.00 mL, and 4.00 mL of 2.00x 10 MKSCN 4. Fill each flask to the mark with 0.10 M HNO, solution. Stopper each flask and invert 5. Measure the absorbance of each solution at a wavelength of 447 nm (the absorbance reterred to in step 1...
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