Sceintists can identify the gene mutated in a bacterial auxotroph by transforming the cells with a...
Sceintists can identify the gene mutated in a bacterial auxotroph by transforming the cells with a genomic library in whihc fragments of the auxotroph's genome have been cloned into plasmids.
Select one:
a. False
b. True
Homework Answers
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Sceintists can identify the gene mutated in a bacterial
auxotroph by transforming the cells with a...
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are...
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are broken down into small DNA fragments. DNA fragments are separated by the process of membrane filtration DNA fragments are cloned into bacterial cells to create a library. Contigs are ordered to create a complete genomic sequence.
A scientist wishes to express a eukaryotic protein in bacterial cells. The gene is cloned along...
A scientist wishes to express a eukaryotic protein in bacterial cells. The gene is cloned along with its promoter region and is inserted into a plasmid. After transforming the plasmid into bacterial cells, protein expression is initiated, but no protein is observed after the cells are lysed. Why? How could this problem be fixed?
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation....
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation. B. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector. C. To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes D. Transformed bacteria are plated onto media containing the appropriate antibiotic and only bacteria containing the plasmid with antibiotic resistance will grow.
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
Please answer these four questions. 6C. A type of plasmid used frequently as a vector in...
Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
Select ways in which genomic instability can arise. over proliferation of cells bacterial infecti...
Select ways in which genomic instability can arise. over proliferation of cells bacterial infections changes in chromosome structure changes in chromosome number mutations in DNA-repair genes
Select ways in which genomic instability can arise. over proliferation of cells bacterial infections changes in chromosome structure changes in chromosome number mutations in DNA-repair genes
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
You have isolated a strain of brewer's yeast (Saccharomyces cerevisiae) in the lab for your second...
You have isolated a strain of brewer's yeast (Saccharomyces cerevisiae) in the lab for your second job at a hip new microbrew (you make a great IPA). You find that this strain ferments more efficiently and adds a superior flavour profile to your brews. You want to clone the strain of yeast and generate a genomic library to determine the genes responsible for this finding. To do so you: 1. Obtain fragments of the whole yeast genome (DNA) through restriction...
colony which one express gene Only correct explanation not just answears pls. how to determine correct...
colony which one express gene
Only correct explanation not just answears pls.
how to determine correct orientation by electrophoresis
CORI -Hindi mal kpn ! Aggi -Hind III Gene CDNA EcoRI 5. G' AAT TC 3 CTTAAG. 5 Бра 5GGT ACC 3 3 CCATGG 5 pMAE2 Avr 11 M5 CCTAGGS 3 GGATCC.5 Arell ACCGGT 3 3.. TGGCCA...5 Amp Hind 1 5: AAGCTT 3 S 3. TTCGAA53 Xma C CGGG.3 GGGCGC 5 You try the following strategies in order to see if...
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are broken down into small DNA fragments. DNA fragments are separated by the process of membrane filtration DNA fragments are cloned into bacterial cells to create a library. Contigs are ordered to create a complete genomic sequence.
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
Select ways in which genomic instability can arise. over proliferation of cells bacterial infections changes in chromosome structure changes in chromosome number mutations in DNA-repair genes
Select ways in which genomic instability can arise. over proliferation of cells bacterial infections changes in chromosome structure changes in chromosome number mutations in DNA-repair genes
You have isolated a strain of brewer's yeast (Saccharomyces cerevisiae) in the lab for your second job at a hip new microbrew (you make a great IPA). You find that this strain ferments more efficiently and adds a superior flavour profile to your brews. You want to clone the strain of yeast and generate a genomic library to determine the genes responsible for this finding. To do so you: 1. Obtain fragments of the whole yeast genome (DNA) through restriction...
colony which one express gene
Only correct explanation not just answears pls.
how to determine correct orientation by electrophoresis
CORI -Hindi mal kpn ! Aggi -Hind III Gene CDNA EcoRI 5. G' AAT TC 3 CTTAAG. 5 Бра 5GGT ACC 3 3 CCATGG 5 pMAE2 Avr 11 M5 CCTAGGS 3 GGATCC.5 Arell ACCGGT 3 3.. TGGCCA...5 Amp Hind 1 5: AAGCTT 3 S 3. TTCGAA53 Xma C CGGG.3 GGGCGC 5 You try the following strategies in order to see if...
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