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14 Which amino acid residue sequence would mostly likely compose a transmembrane segment of a protein?...
13 14 Search 13 How will a mutation in the active site df an enzyme that...
13 14
Search 13 How will a mutation in the active site df an enzyme that reducing substrate binding affect enzyme parameters? OS (2 points) Reduce Vm Reduce Km Increase km Reduce Vm and km 14 Which amino acid residue sequence would mostly likely compose a transmembrane segment of a protein? (2 Points) VLVAVLESFLLLSL GRRHLGFEATFFQ GRKKRRORRRPQ NVFKGNTISOKIS 15 What would be observed if purified IgG was boiled and not reduced and then applied to SDS- PAGE (2 points)
Hydropathy analysis of a membrane protein's amino acid sequence predicts the protein's transmembrane segments and orientation...
Hydropathy analysis of a membrane protein's amino acid sequence predicts the protein's transmembrane segments and orientation in the membrane. It is necessary to confirm those predictions with experimental analysis. One experimental approach for plasma membrane proteins is to use the protease trypsin to digest extracellular domains of these proteins. (Trypsin is hydrophilic and cannot cross the plasma membrane to enter the cell.) When added to cells, trypsin digests the hydrophilic portions of plasma membrane proteins exposed outside the cell into...
The following is a portion of the amino acid sequence of human GLUT1 purified from red...
The following is a portion of the amino acid sequence of human GLUT1 purified from red blood cells. Glucose is also shown for your convenience. 147 151 161 171 181 vspt alrgalgtlh qlgivvqili aqvfgldsim gnkd A. An internal 23 amino acid region of the sequence above is the 5th transmembrane domain of GLUT1. The beginning of the sequence is an intracellular loop. The end of the sequence is an extracellular loop. Indicate where these regions might be in the sequence. Explain how you derived at your hypothesis....
Question 5 Bio206 Homework 6 Amino Acids and Proteins Organic Chemistry II Due May 6, 2017 1. Which amino acid is least likely to be found in a natural protein? CH20H NHz CHs IV 2. A pentapeptide has...
Question 5
Bio206 Homework 6 Amino Acids and Proteins Organic Chemistry II Due May 6, 2017 1. Which amino acid is least likely to be found in a natural protein? CH20H NHz CHs IV 2. A pentapeptide has the molecular formula: Asp, Glu, His, Phe, Val. Partial hydrolysis of the pentapeptide gives: Val Asp, Glu His, Phe Val, and Asp Glu. What is the amino acid sequence of the pentapeptide? 3. When the pentapeptide below is heated first with 2,4-dinitrofluorobenzene...
1. The following amino acid sequence is observed in an alpha-helical segment of a polypeptide: L D E N I K R N A Q L V E...
1. The following amino acid sequence is observed in an alpha-helical segment of a polypeptide: L D E N I K R N A Q L V E Q Q I R What pattern, if any, seems to characterize this sequence? Explain why this pattern might be occurring in terms of the 3D structure of the protein. 2. Indicate the probable location of the following amino acid residues in a native globular protein. a) Asp b) Phe c) Met d)...
Which sequence of amino acids would likely be found in the interior of a globular protein?...
Which sequence of amino acids would likely be found in the interior of a globular protein? GMWS IEDP HEKR DEAR Which of the following reactions is spontaneous in the forward direction when the reactants are my present in equirnolar amounts ? Glyceraldehyde 3 phosphate e Dihydroxyacetone phosphate creatine phosphate . ADP ATP creatine ADP. 1,3 bisphosphoglycerate 3 phosphoglycerate + ATP glycerol 3-phosphate + ADP = ATP glycerol NADH+ + H+ oxaloacetate NAD+ + malate NAD+ + pyruvate + COA NADH...
In addition to the primary amino acid structures of been insulin discovered by F. Sanger, the...
In addition to the primary amino acid structures of been insulin discovered by F. Sanger, the structures of other animal insulins have been determined. The only differences among these insulins lie in a small amino acid sequence adjacent to a cysteine residue. Some of the differences for this sequence are as follows: Beef: ala-ser-val Sheep: ala-gly-val Pig: thr-ser-ile Horse: thr-gly-ile 1) Using a codon chart, could single nucleotide changes account for the observed substitutions in the first ...
The following amino acid sequence is observed in an alpha-helical segment of a polypeptide: L D E N I K R N A Q L V E Q...
The following amino acid sequence is observed in an alpha-helical segment of a polypeptide: L D E N I K R N A Q L V E Q Q I R What pattern, if any, seems to characterize this sequence? Explain why this pattern might be occurring in terms of the 3D structure of the protein.
Consider the following amino acid sequence, found as part of a larger protein: Pro-Gly-Asp-Val-Gln-Phe-Asp-Ile-Arg-Ala-Asp-Gly What kind...
Consider the following amino acid sequence, found as part of a larger protein: Pro-Gly-Asp-Val-Gln-Phe-Asp-Ile-Arg-Ala-Asp-Gly What kind of structure do you expect this peptide segment be a part of? Where on the protein is this likely to occur?
Table 1: Partial RPE65 protein sequence (amino acids 41-60) for the 9-year-old LCA patient. Unmutated Protein...
Table 1: Partial RPE65 protein sequence (amino acids 41-60) for the 9-year-old LCA patient. Unmutated Protein Sequence Patient's Allele 1 Protein Sequence Patient's Allele 2 Protein Sequence START...Ser-Leu-Leu-Arg-Cyc-Gly-Pro-Gly-Leu-Phe-Glu-Val-Gly-Ser-Glu-Pro-Phe-Tyr- His-Gly...STOP START...Ser-Leu-Leu-Gin-Cyc-Gly-Pro-Gly-Leu-Phe-Glu-Val-Gly-Ser-Glu-Pro-Phe-Tyr- His-Gly...STOP START...Ser-Leu-Leu-Gin-Cyc-Gly-Pro-Gly-Leu-Phe-Glu-Val-Gly-Ser-Glu-Pro-Phe-Tyr- His-Gly...STOP Table 2. Partial RPE65 protein sequence (amino acids 61-70 and 291–300) for the 11-year-old LCA patient. Unmutated Protein Sequence Patient's Allele 1 Protein Sequence Patient's Allele 2 Protein Sequence START...Phe-Asp-Gly-Gln-Ala-Leu-Leu-His-Lys-Phe...lle-Ala-Asp-Lys-Lys-Arg-Lys-Lys- Tyr-Leu...STOP START...Phe-Asp-Gly-Gln-Ala-Leu-Leu-Tyr-Lys-Phe...lle-Ala-Asp-Lys-Lys-Arg-Lys-Lys- Tyr-Leu...STOP START...Phe-Asp-Gly-Gln-Ala-Leu-Leu-His-Lys-Phe...lle-Ala-Asp-Lys-STOP Source: Data from Russell et al. (2017). Use Tables 1 and 2 to...
13 14
Search 13 How will a mutation in the active site df an enzyme that reducing substrate binding affect enzyme parameters? OS (2 points) Reduce Vm Reduce Km Increase km Reduce Vm and km 14 Which amino acid residue sequence would mostly likely compose a transmembrane segment of a protein? (2 Points) VLVAVLESFLLLSL GRRHLGFEATFFQ GRKKRRORRRPQ NVFKGNTISOKIS 15 What would be observed if purified IgG was boiled and not reduced and then applied to SDS- PAGE (2 points)
Question 5
Bio206 Homework 6 Amino Acids and Proteins Organic Chemistry II Due May 6, 2017 1. Which amino acid is least likely to be found in a natural protein? CH20H NHz CHs IV 2. A pentapeptide has the molecular formula: Asp, Glu, His, Phe, Val. Partial hydrolysis of the pentapeptide gives: Val Asp, Glu His, Phe Val, and Asp Glu. What is the amino acid sequence of the pentapeptide? 3. When the pentapeptide below is heated first with 2,4-dinitrofluorobenzene...
Which sequence of amino acids would likely be found in the interior of a globular protein? GMWS IEDP HEKR DEAR Which of the following reactions is spontaneous in the forward direction when the reactants are my present in equirnolar amounts ? Glyceraldehyde 3 phosphate e Dihydroxyacetone phosphate creatine phosphate . ADP ATP creatine ADP. 1,3 bisphosphoglycerate 3 phosphoglycerate + ATP glycerol 3-phosphate + ADP = ATP glycerol NADH+ + H+ oxaloacetate NAD+ + malate NAD+ + pyruvate + COA NADH...
Table 1: Partial RPE65 protein sequence (amino acids 41-60) for the 9-year-old LCA patient. Unmutated Protein Sequence Patient's Allele 1 Protein Sequence Patient's Allele 2 Protein Sequence START...Ser-Leu-Leu-Arg-Cyc-Gly-Pro-Gly-Leu-Phe-Glu-Val-Gly-Ser-Glu-Pro-Phe-Tyr- His-Gly...STOP START...Ser-Leu-Leu-Gin-Cyc-Gly-Pro-Gly-Leu-Phe-Glu-Val-Gly-Ser-Glu-Pro-Phe-Tyr- His-Gly...STOP START...Ser-Leu-Leu-Gin-Cyc-Gly-Pro-Gly-Leu-Phe-Glu-Val-Gly-Ser-Glu-Pro-Phe-Tyr- His-Gly...STOP Table 2. Partial RPE65 protein sequence (amino acids 61-70 and 291–300) for the 11-year-old LCA patient. Unmutated Protein Sequence Patient's Allele 1 Protein Sequence Patient's Allele 2 Protein Sequence START...Phe-Asp-Gly-Gln-Ala-Leu-Leu-His-Lys-Phe...lle-Ala-Asp-Lys-Lys-Arg-Lys-Lys- Tyr-Leu...STOP START...Phe-Asp-Gly-Gln-Ala-Leu-Leu-Tyr-Lys-Phe...lle-Ala-Asp-Lys-Lys-Arg-Lys-Lys- Tyr-Leu...STOP START...Phe-Asp-Gly-Gln-Ala-Leu-Leu-His-Lys-Phe...lle-Ala-Asp-Lys-STOP Source: Data from Russell et al. (2017). Use Tables 1 and 2 to...
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