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a. Identify the type of inhibition occurring. Explain how you know. b. Describe the difference or similarity between the Vmax
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Answer #1

This plot is called Lineweaver Burk and it is the perfect graph to visualize inhibition type in an enzymatic reaction. The point at which the line crosses the Y axis is equal to 1/Vmax, and the point where is crosses the X axis is equal to -1/Km. This means we can completely visualize any change in Vmax and Km with this graph.

When we add indol to the reaction we can see how Vmax is not altered but Km actually IS. This pattern is related to COMPETITIVE inhibition, and this means indol interacts with the enzyme by binding to the same active site used for the substrate, that is why it is "competing" with the substrate. The same Vmax can be reached but you need a lot more of substrate concentration to reach it, that is why Km is increased.

a) Competitive inhibition

b) Vmax stays the same while Km increases

c) Indole binds to the active site in the enzyme (not allosteric sites)

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