Describe the evidence for chemiosmotic coupling in mitochondria.
The chemiosmotic hypothesis was proposed by Peter Mitchell.
It states that a proton-motive force was responsible for the synthesis of ATP.
In this, H+ are be pumped across the inner mitochondrial membrane as electrons went through electron transfer chain.
This result in a H+ gradient with an lower pH in the intermembrane space and a higher pH in the matrix of the mitochondria.
Evidence supporting the chemiosmotic hypothesis is
Describe chemiosmotic coupling model and its relationship with pmf. Discuss key evidence supporting the model
26) Briefly describe, using words or simple diagrams, the chemiosmotic theory for coupling oxidation to phosphorylation in mitochondria. (5 pts)
1. Explain chemiosmotic coupling and its relation to oxidative phosphorylation: 2.Illustrate and describe the mechanism of ATP synthase conversion of gradient energy to mechanical energy to chemical energy. 3. Explain common sense feedback regulation of metabolic pathways.
Describe three pieces of evidence that mitochondria and evolved from symbiotic mutualisms between a eukaryote (or eukaryote ancestor) and bacteria.
what evidence is there that mitochondria and chloroplasts originated from ancient prokaryotes and why?
16. What do the mitochondria of parabasalids and diplomonads have in common? Describe these mitochondria and explain how the organisms conduct cellular respiration.
Outline TWO pieces of evidence for the endosymbiotic hypothesis for the origins of chloroplasts and mitochondria in eukaryotic cells. (4 marks)
Which of the following are generally viewed as evidence that mitochondria and chloroplasts are derived from single celled organisms (select all that apply)? Both miochondria and chloroplasts divide autonomously using a mechanism that resembles binary fission Mitochondrial and chloroplast genomes both exhibit sequence similarity to extant single celled organisms Mitochondria and chloroplasts are both capable of persisting indefinitely outside of eukaryotic cells Both mitochondria and chloroplasts have their own circular genomes
What evidence supports the contention that the proton gradient drives ATP formation in mitochondria? Give at least 2 lines of evidence.
Describe the differences between substrate-level phosphorylation and chemiosmotic (oxidative) phosphorylation as mechanisms of ATP formation. Where in a prokaryotic cell does each occur? What are the substrates that are used to form ATP? What is the source of energy for ATP synthesis?