Question

QUESTION 1 Match each E10 reaction component with its role. A. substrate Nitrophenyl phosphate stops reaction and allows absorbance of product to be B. measured Nitrophenol Acid phosphatase C. makes pH optimal for enzyme activity 1.5% KOH D. enzyme E. product QUESTION 2 This experiment assays the reaction rate of wheat germ acid phosphatase at a temperature of [a]°C.

Answer 1

Acid DATA enzyme Assan u used to measure the 2, 4. phosphatase Ansa It experment which is used to determail the amount of nitrophenal produced. It is are vital for sending enzyme kinelles & enzyme inhibition Nitropheryl phosphate => substrate Nitrophenol Product 3. Acid phosphatare Enzyme 1.5% KOH => Stops reaction and allows absorbance of product to be measured. Substrate Nitrophenyl phosphate thyo Phosphate + (Colorless in alkaline solutions) Nitrophenol (Yellow in alhodit The Solutions) purpose of the Arssay is to detamine the amont of nitrophand obtained in the reaction. Were and standard currere of OD 410 vs n molelul of Witrophenol grounded which is then converted into concentration The acid phosphate assays. This expernment as the reaction rate of what acid phosphatase at a temperature of 3700 of nitrophend 2 Ans geam