Question

You want to determine whether a person has a certain mutant gene. The process involves using a primer and a heat-stable DNA polymerase. This process is: Select one: a. translation b. restriction mapping. c. PCR. d. site-directed mutagenesis. e. transformation

Answer 1

You Page 1 want to determine a person has a certain mutant The pro cess involves a primer and a heat -stable polymerase. This process gene DNA per Polymerase chain reaction (PER) is a method Used to brapidly make millions to widely

billions is now a common in medical including mutation, Page 2 of copies of a specific DNA sequence. Per and often indispensable technique used laboratory for broad variety of application biomedical research, detection of particular criminal forensies. Many forms involve alteration sto PER based test to study these therapy regimens be Indivisually customized and caneer oncogenes. By using mutations a patient. can to to understand how proteins PER can be used to create mutant genes with mutations mutations chosen by seientists at coill. These can be chosen in order accomplish their functions and to change or improve improve protein function. Primers PER imploys two main reagents single stranded DNA fragments that are a complementary sequence to the target DNA region) and a DNA polymerase such as Taq polymer- (which are short heat stable ase. Correet because : Other options are not mathod ased to map Translation: Translation is the process in which protein is synthesized from mRNA in vibo somes. Restriction mapping: Restriction mapping an unknown segment of DNA by breaking it into pie ces and then identify lo eation the breakpoints. This method involves restriction enzyone cohich can cut digest molecules at specific Sequences called lestriction sites. the or DNA

- 3 Page @ site - directed mutagenesis It is a method to create specific targeted changes in double stranded plasmid DNA. They a heat stable DNA polymerase primer is not directly needed in this methat. Per is done as a step site directed mutagenesis. RSD OY le It is transformation > the genetic alteration of a cell resulting from direct uptake and in corporation of endogenous genetic material from its surroundings through the cell membrane. can result Base substitution and frame shift mutation in the formation of premature stop estons. True tides A frame shift mutations a genetic mutation caused by insertions and or dele Hone of a number of nucleo- in a DNA and can change the reading frame. As a result a co completely different translation of protein Gecurs. The frame shift mutation will also atter can produces a termination codon or stop eodon. Base substitutions matation A nonsense mutation refers to a base substitu Hons in which nucleotide transforms the codon into a stop codon. Such a leads to change a pre mature termination of translation, the changed
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