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You want to determine whether a person has a certain mutant gene. The process involves using...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS WELL. THANKSSSSSSS 1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
can you guys please give me the correct answers and explain why? 3. Which of the following statements is CORRECT? A. Exposure of E.coli to UV light greatly increases the frequency of cytosine deamination B. Mutagens that intercalate into the double helix lead to the formation of thymine dimers. C) Oxidative damage to DNA usually leads to the formation of frameshift mutations. D. Some alkylating agents are mutagenic because they chemically modify amino acids in the active site of DNA...
MUN for the rapid SA can be enormously benehciarlo NS FOR FURTHER REVIEW be found in the answer section at the back wers only after you have attempted to solve th at the back of this d to solve the ques- Answers to these questions can be found in the study guide. Refer to the answers only after you tions ON JONr Own. Multiple Choice 1. DNA genomes are found in: a. All organisms and all viruses b. All organisms,...
n 13 You have found a mutant that has a G-protein that can only bind GDP and cannot bind GTP. Which of the following might you expect in the mutant cells? red ed out of ag question Select one: a. The secondary messenger would be made constantly. b. The cells would have more energy availability due to higher GTP binding. c. The secondary messenger would not be made. O d. The primary messenger would not be able to bind to...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...