Question

In humans, Wiskott-Aldrich syndrome is characterized by abnormal immune system function (immune deficiency) and a reduced ability to form blood clots. Mutations in a single gene, WAS, gene confer Wiskott-Aldrich syndrome. You study it and use CRISPR-Cas9. Describe how you would design the constructs for this experiment in which you repair the mutations in WAS, conferring normal immune system function and blood clotting. Include all relevant constructs you’d need to make, and what model organism you would use