Question

Which statement about polymerase is false? Select one: a. The polymerase reaction is unique because it was the first example of a biosynthetic reaction using a template b. DNA synthesis is a highly favorable reaction due to a 19 kJ/mol generated in the subsequent hydrolysis reaction of pyrophosphate by pyrophosphatase. c. Processivity is defined as the average number of nucleotides added before the polymerase dissociates. d. DNA polymerase requires a primer with a free 3-hydroxyl group. e. The DNA polymerase uses both Mg2+ to stabilize the beta and gamma of pyrophosphate.

Answer 1

Solution: The correct option is option B Hydrolysis of the pyrophosphate to two molecules of inorganic phosphate by the pyro phosphatases present in all cells yields a AG of -30 kJ/mol, and by coupling these two reactions the cell can provide a strong thermodynamic pull in the direction of polymerization, with a net AG of - 28 kJ/mol.