Question

Allosteric effectors on the rate of oxidative deamination catalysed by the enzyme glutamate dehydrogenase 1. The rate of disappearance of NADH (v) is shown in blue (Reaction 11, by following the absorbance at 340 nm: so the graph has a negative slope. 2. The rate is also tracked in the presence of GTP (in red; Reaction 2) and ADP (in black: Reaction 3) 1. From the values for the reaction rate shown in Table 2, , determine the nature of the allostery (positive or negative) for GTP and ADP and why you have come to this conclusion. Table 2: Comparison of reaction velocities for Reactions 1-3 no.min" per 450 ul of enzyme (ADP) 0.4208 effectors of GDH? For each effector, classify if it is a positive effector or a negative effector (inhabitors) of GDH activity. Also provide a reason why. Hint compare the reaction rates for Rescns 2 and with tha 50 0000 9000

Question: Did you find that GTP or ADP acted as effectors of GDH? For each effector, classify if it is a positive effector or a negative effector (inhibitors) of GDH activity. Also provide a reason why.

Answer 1

The achvit ofGop is regulukd b oetos that ontrol thúr abili dtoansh diphosphate. GTP s acHve and se along the eembtane surfuo , achvate target ρ70tuhs,ự Some tine inhibit best effector equlafed bt tvimeric G proteins GOn'a chút .1 s subject to complex γegul ahon by negative CGTP,