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QUESTION THREE (20 MARKS) The enzyme malate dehydrogenase (MDIH) catalyses the following reaction: Malate +NADoxaloa...
First question: Which type of enzyme fron table 11-2 catalyzes the following reactions (a) 600 c=0 CH + NADH + H - 500- + NAD HO-- CH, COO H-CH(CH HỘI NH ADP + P 5. On the free energy diagram shown, label the intermediate(s) and transition state(s). Is the reaction thermodynamically favorable? 6. Urcase, the first enzyme to be crystallized, is inhibited in the presence of Hg, Cd, or Co ions What does this information suggest about the catalytic mechanism...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
Question 5 Your friend is an amateur mushroom hunter. He accidentally eats an Amanita phalloides "Death Cap" mushroom and becomes very ill. You recall from university that this mushroom is toxic because it contains a aminitin, a noncompetitive inhibitor of RNA polymerase. Which of the following hypothetical treatments (if they were possible) would NOT help your friend? Select all best answers for full credit Injecting more RNA polymerase into his cells. Injecting a drug that binds the enzyme-binding region a...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...