➢Advantages of Column chromatography
1.It can be used in both analytical and preparative
applications.
2.It is used to determine the number of components of a
mixture.
3.It is also used to separate and purify substantial quantities of
those components for subsequentanalysis.
➢Disadvantages of Column Chromatography
1.Properly setting up the column requires some technical skill and
manual dexterity.
2.It is very time consuming and tedious, especially for large
samples.
3.Collecting vessels must be frequently switched and solvent levels
need to be topped up.
20 marks Discussion Dry column chromatography. State 2 advantages and 1 disadvantage over conventional column chromatogr.
1) In a column chromatography procedure, if two compounds were eluted in the same fraction, how would you separate them? 2) why is it important to not let the column dry out?
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC and TLC operations conducted concurrently? Explain. 3. Name a few packing materials used in packing columns in Column Chromatography operations. 4. Explain the role of eluting solvent(s) in column chromatography procedures. 5. When performing column chromatography, which of the following compounds would you expect to elute first: Ferrocene of acetylferrocene? Explain why.
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase? 3. What is the purpose of the cotton (glass wool) in column chromatography? 4. What is the purpose of the sand? 5. Why is it important to prevent the column from drying? 6. What can happen if you disrupt your even layer of silica gel? 7. Which compounds come off the column first, polar or nonpolar? 8. Keyon synthesized acetylferrocene (orange) in organic chemistry...
1) Describe the differences between dry packing of the column like in the procedure and the wet method described in the video. Give two advantages and two disadvantages of each method for packing. 2) In the lab manual and the video tutorial, silica gel is used as the preferred stationary phase. Can you name two more options for a stationary phase? Discuss two advantages and two disadvantages compared to silica gel.
Describe the differences between paper chromatography and TLC. Give two advantages and two disadvantages of each method for analysis. In order to prepare for a column chromatography separation of 3 ink dye components, you have run a TLC experiment with three possible solvent mixtures. These are the results: Acetone:Hexane in 1:1 ratio gave the following distances: solvent=4.2cm, pigment #1=3.8cm, pigment #2=1.7cm, and pigment #3=1.3cm Acetone:Hexane in 2:1 ratio gave the following distances: solvent=4.3cm, pigment #1=3.8cm, pigment #2=2.1cm, and pigment...
discuss 1- the advantages and disadvantages of ion exchange chromatography( IEC ) compared to reversed phase liquid chromatography and use specific examples from the literature. 2- specify some samples that you could separate with IEC that you couldn't separate with reverse phase liquid chromatography .
1. List any 3 advantages and 1 disadvantage of surface mount devices (SMDs). 2. List the principles used in PCB shielding. 3. What are 2 main principles of cable routing?
QUESTION ONE (20 Marks) 1.1 What are the advantages of observation and its limitations as a method of collecting (6 marks) nformation? 1.2 State TWO (2) advantages of primary data 1.3 Explain any TWO (2) differences between a bar chart and histogram. 1.4 The capacities (in millions of litres) of each of the four major storage dams that supply (2 marks) (2 marks) the water requirements of Cape Town are shown in the table 1.1 below Storage Dam Capacity (MI)...
Question 2 (1 point) In liquid chromatography the stationary phase carry each substance along the column. True False
1. What is the function of a diode bridge rectifier? State one disadvantage of the diode bridge rectifier and describe a way of overcoming this. You may use words or sketch the circuit. [6 marks] 2. What is the function of a buck converter? Give an example application. Identify two limitations of this circuit. [6 marks]