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discuss 1- the advantages and disadvantages of ion exchange chromatography( IEC ) compared to reversed phase...

discuss 1- the advantages and disadvantages of ion exchange chromatography( IEC ) compared to reversed phase liquid chromatography and use specific examples from the literature. 2- specify some samples that you could separate with IEC that you couldn't separate with reverse phase liquid chromatography .

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Answer #1

Advantages of IEC:

It is a non-denaturing technique. It can be used at all stages and scales of purification

An IEX separation can be controlled by changing pH, salt concentration and/or the ion exchange media

It can serve as a concentrating step. A large volume of dilute sample can be applied to a media, and the adsorbed protein subsequently eluted in a smaller volume

It offers high selectivity; it can resolve molecules with small differences in charge.

Disadvantages:

costly equipment and more expensive chemicals.

turbidity should be below 10ppm.

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