Question

Format 192.168.12 Tools Table Window Help o 5 0 Home Insert Draw Design Layout Times New R. 12 . A- A B TUXX google.cc Oxidation and Reduction Enzymes References Mailings Review View A A A TEE . . . .A .A . NYCD Paste A. . + . . Some media are designed to give multiple test results. Examples include: 1. K ler's Iron agar slant which determines Glucose and Lactose fermentations and production of Hydrogen sulfide 2. SIM which determines production of Hydrogen Sulfide, Indole production and Motility 3. Russel's Double sugar agar slant which determines Glucose and Lactose fermentations Kligler' Tron agar slant: Contains 1% glucose, 1% lactose, peptone, ferrous salts and phenol red as pH indicator The Lactose is in the slant area of the medium and Glucose at the bottom section of the medium and can detect lactose fermentation and glucose fermentation from the color of the pH indicator phenol red in those areas. The medium will also detect Hydrogen Sulfide production from the breakdown of the sulfur-containing amino acid Cysteine in the medium based on released hydrogen sulfide reacting with famous salts in the medium to form casily detectable black precipitate of ferrous sulfide. Breakdown of the amino acid cysteine occurs from the activity of the enzyme Cysteine desulfurase The medium is inoculated by streaking the slant area and stabbing the bottom part of the tube Bacteria Bottom Glucos Slant. Lactose HS Interpretation produced 1 Profeus vulgaris Yellow Red Black ppt 2 Escherichia coli Yellow Yellow No black T 2 3 Staphylococcus Yellow Red aureus 4 Salmonella Yellow Red ppt Questions: 1. Fill in your interpretation of the above results in the blank spaces numbered 1-4 2. Which of the test bacteria above tested positive for Cysteine desilfurasg enzyme? Explain your answer. NEW ME 2 SIM (Sulfide Indole Motility): A multiple test medium that detects the production of hydrogen sulfide from the activity of cysteine desulfurase enzyme on the amino acid cysteine, and indole from the activity of the enzyme tryptophanase on the amino acid tryptophan. Also determines if the organismis motile or not, thereby indicating whether the test bacteria have flagella or not. The Test Medium is a semi-solid medium to accommodate motility and inoculated by stabbing. It contains ferrous salts to react with hydrogen sulfide produced by the degradation of cysteine to form black precipitate of ferrous sulfide. Also contains tryptophan, the breakdown of which will produce indole and can be detected by Koyas's Tearcot atteicubation.. RESULTS Page 9 of 10 3827 words English (United States) Focus

Answer 1

1. Interpretation chart is alright!

2. Cystinuria a disease where urine becomes rich in amino acid, cysteine. And, in this condition, Urinary tract becomes rich with cysteine.

Proteus vulgaris is responsible for causing urinary tract infection. This organism tends to grow in the urinary tract.

This is the reason, why Proteus vulgaris produce cysteine desulferase, so that pyruvate can be produced easily, just by removing the sulfhydryl and amino groups from cysteine, present in leftover urines in urinary tract.

Thank You!