Question

D e eest. Results are determined by one of two ways: Ord O Directly observe First perform a chemical test using media for results OR reagents. THEN observe media for results For example read through Physical Factors: Atmospheric Oxygen Requirements. (you do not do this test for your unknown). Pene 04. 1 pt. What is it you are testing for with this test? This should be a general answer, not a list of the five types of metabolic pathways. Omnou FM CD Q5. Ipt. What type of agar is used? (We do not do this assay because it involves molten agar which is hard to keep on hand) 06. Ipt. How are the results determined? Choose one of the two ways described above. You will have to use the book AND the handouts you picked up in lab for the following questions: Use the word bank to respond to each prompt below: Blood agar hydrogen peroxide urea broth indole carbohydrate fermentation broths red hemolysins bright pink SIM agar carbon dioxide MSA DNase agar phenol red yellow acid production Catalase Bile esculin agar amylase nitrate broth Simmons citrate agar 7.5% salt broth nitrate oxidase dark blue urca EMB methyl green MR-VP TSA Q7. Hydrogen peroxide is released by white cells to help kill bacteria. Which enzyme do some bacteria have to break down hydrogen peroxide? 08. Phenol red turns which color indicating carbohydrate fermentation with acid production? 09. The plate used to determine oxidase activity will also be used to determine if a bacterium makes another enzyme called: Q10. Two types of media detect lactose fermenting bacteria Q11. DNase agar contains this indicator (changes color) Q12. This media is used for the vogues proskauer test Q13. Blood agar is used to detect these molecules Q14. Urea broth is used to detect the breakdown of urea to: Q15. This media determines fermentation of glucose with the production of acid end products (stabilized) Q16. SIM agar determines if a bacterium can produce: Q17. Which color is produced by a positive oxidase test? Q18. Most gram negatives cannot grow in this broth since it is very hypertonic 019. Which of the following detects the ability to reduce nitrate? 020. This media detects motility and the breakdown of tryptophan to indole

Answer 1

7. Catalase is an enzyme that has been present in aerobic, aerotolerant and facultative aerobic bacteria which breakdown hydrogen peroxide to water and hydrogen. It protects them from the harmful Reactive Oxygen Species (ROS).