Question

Test	Purpose	Reagents	Observation	Results
Gram	To determines the identify the bacteria as a gram positive or a gram negative.	Crystal violet, iodine, Alcohol ,safranin	Pink rods	Gram negative rods
Oxidase	To determine the presence of cytochrome oxidase enzyme .	Oxidase paper
Lactose	To determine if the bacteria can ferment carbohydrate .	None
Indole Test	To determine the bacteria’s ability to decompose amino acid tryptophane to indole.	Kovac added to1ml of trypotone broth
Optimum Temp	To determine the bacteria’s ability to live in a human body .
Gelatin liquefaction	To determine the bacteria’s ability to produce glatinase enzyme.
Starch	To determine if the bacteria uses starch.
Casein	To determine the bacteria’s ability to digest casein .
Litmus milk	To determine the bacteria’s ability to ferment lactose.
Phenylalanase	To determine the bacteria’s ability to produce the enzyme deaminase.
Catalase	To determine the bacteria’s ability to produce catalase.
Urease	To determine the bacteria’s ability to split urea by the production of enzyme urease.
Nitrate Reduction	To determine the bacteria’s ability to reduce nitrate reductase.
Citrate utilization	To determine the bacteria’s ability to utilize sodium citrate.
Methyl Red	To determine the bacteria’s ability to produce of sufficient acid during the fermentation of glucose.
Oxygen requirements	To determine whether the bacteria is anaerobic or aerobic .
Mannitol	To determine the bacteria ability ferment carbohydrates.
Motility	To determine the bacteria’s ability to move by it’s self by using flagella .
Endospore	To differentiate bacterial spores from other vegetative spores .
Gelatin stab growth pattern	To determine the bacteria’s ability to produce gelatinases,differentiate species.
Morphological characteristics	To determine the bacteria’s size, shape and arrangement.
Culture characteristic	To determine bacteria’s pigmentations in certain temperatures, colony size.
V-P	To determine the bacteria’s ability to produce acetylmethyl carbinol.
Glucose Test	The bacteria’s ability to ferment the glucose.
Fat Test