Question

Medium/test used for/ determines indicator / key ingredients positive result negative result Comments/ additional info quadrant streak Gram stain motility agar Eosin methylene blue agar (EMB) SIM Nm multi test medium (3 results in one medium) Brewer's plate in GasPak Fluid thioglycollate (FTM) Citrate slant carbohydrate test media (list the ones tested) phenylalanine agar urea agar or broth MR/VP broth MR test: VP test: nitrate broth catalase test oxidase test casein agar gelatin deep lysine iron Pseudo. F agar & Pseudo. P agar temp specific incubations

Answer 1

MEDIUM/ TEST	USED FOR / DETERMINES	INDICATOR	POSITIVE RESULT	NEGATIVE RESULT	ADDITIONAL INFORMATION
Quadrant streak	isolate the colonies of different group of bacteria
gram stain	differentiate gram +ve and gram -ve bacteria	crystal violet stain; counterstain	purple color(gram +ve)pink color(gram -ve)	no stain	differentiate bact. by their physical and chemical properties of cell wall by staining process.
motility agar	differentiate between motile and non motile bacteria	agar	spreading throught out the medium	present only the area of incubation	non motile bacteria grow only in soft agar tube.
eosin methylene blue agar	identify gram -ve bacteria that fermet lactose	blend of two stain (eosin &methylene blue	nucleated colonies with purple & black	colorless colonies	lactose fermentation lower the ph that increases dye absorpation
SIM	1.sulfide 2. indole 3. mobility	sodium thiosulphate and peptone including tryptophan	black color( + for sulfur reduction) red color (+ for indole production) growth all over the tube ( motile)	no color change
brewers plate in gaspak	cultivating anaerobic and microaerophilic bacteria	brewer anaerobic agar with sodium thioglycollate	show growth in absence of o2	no growth
fluid thioglycollate	cultivation of areobic , anaerobic & microaerophillic organism	sodium thioglycollate and agar	aerobes(O2 conc highest) bact gathered on top of tube	anaerobic( O2conc lowest) gathered in bottom	microaerophilic gathered in upper part but not in top
citrate slant	differentiating the gram + bacteria	citrate	bromothymol indicator turn green to blue color in ph > 7.6	no color change if ph < 6.9	bact. use citrate and form bicarbonate and carbonate which increases the ph.
carbohydrate test media	classify bact. fermenter gas and acid production & non fermenter	carbohydrate source	acid production (phenol red indicator turn into yellow) gas production ( gas bubble in tube)	no change in color of phenol red indicator no gas bubble form in test tube
phenylalanine	to determine the ability of organism to deaminate tophenylalanine to phenylpyruvic acid	phenylalanine	green color formed	no green color formed
urea agar	differentiate enterobacterieceae