Question

How can I briefly describe each of the method used to create transgenic plantst a) Protoplast fusion b) leaf fragmentation c) antisense RNA d)Ti plasmids

Answer 1

a) Protoplast fusion - Genetic modification by fusing the protoplast of two different species of plants to obtain a somatic hybrid or transgenic plants . It can be done either by spontaneous fusion or induced fusion.

b) leaf fragmentation : An asexual mode of reproduction by fragmenting leaves which clones into a newly formed mature and new plant.

c) antisense RNA : Antisense RNA strand is that particular strand(single) of RNA which is complimentary to the mRNA or messenger RNA during the process of transcription

d)Ti plasmids: Ti plasmid or Tumour plasmid is the plasmid used to induce genetic materials to plants to create a transgenic plant. It is a part of genetic equipment induced with the help of Agrobacterium rhizogenes and Agrobacterium tumefaciens.