Homework Answers
DNA is negatively charged. If you run DNA sample in a gel under constant voltage, the smaller fragments will migrate faster whereas larger fragments migrate slowly.
Note: The electrophoretic migration velocity (
up=μpE. E is constant
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Please answer several sentences
Describe how the size separation of DNA occurs in capillary electrophoresis (
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively charged (with a charge of two electrons per base pair). When you “run the gel” you are generating an electric field by connecting anodes and cathodes at the ends of the gel. This causes the negatively charged DNA segments to move towards the positive electrode. After running the gel, smaller DNA segments have moved farther from the...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
What is the purpose of gel electrophoresis? How is size related to movement through a gel?...
What is the purpose of gel electrophoresis? How is size related to movement through a gel? What is a DNA ladder? Why is it important in gel electrophoresis and how is it used? (note: a ladder is also called a “standard”) What are two indications one can look for to be certain the gel electrophoresis is occurring? What are two strategies to improve the resolution of DNA bands?
help! 14. Which of the following factors does effect on DNA fragments separation during electrophoresis a....
help!
14. Which of the following factors does effect on DNA fragments separation during electrophoresis a. Size b. Electrical charge c. Nucleotides sequences d. All of the above Match the following with q 15-18 a. A necessary cofactor for DNA polymerization b. To break cell membrane open c. Confirm plant-based sample d. Detection of GMO e. Cell membrane lysis 15. Salt solution and heat 16. Primer of CaMw promoter 17. Primer of Nopalin Synthetase virus terminator 18. Magnesium chloride 19-...
Refer to the picture included in question 7 for this question. Please explain how gel electrophoresis...
Refer to the picture included in question 7 for this question.
Please explain how gel electrophoresis separates the DNA fragments
that are produced by the Sanger technique. Include the following
elements within your explanation (not necessarily in order of how
you should place them in your answer):
electric current and charge
size of DNA molecules
DNA samples
gel
wells
and buffer solution.
I I III I
b. (4 pt) Describe how active 6mA DNA demethylation occurs.
b. (4 pt) Describe how active 6mA DNA demethylation occurs.
Answer all questions: C10) 1. You were given to study a linear DNA sample by agarose...
Answer all questions: C10) 1. You were given to study a linear DNA sample by agarose gel electrophoresis. You were also given a standard DNA size maker. After electrophoresis and staining of DNA, you found several bands in standard DNA size marker (M) and two bands in sample (S) DNA. Size of each standard DNA molecule and their distance from the origin of electrophoresis was provided (see picture below). ) Draw a standard curve using the graph paper provided. Make...
Question 5 The size of a piece of DNA can determine how fast or slow it travels during gel electrophoresis. True...
Question 5 The size of a piece of DNA can determine how fast or slow it travels during gel electrophoresis. True False Question 6 Radioactive probes attach to the complimentary base during some DNA lab processes. For instance a C would attach to a G and a T would attach to an A (nucleic acids). True False Question 7 How could/might all of this type of information at some point in time impact you personally (or your family members)? (Consider...
Please answer your questions in paragraphs of six to ten sentences 1. Several types of LED...
Please answer your questions in paragraphs of six to ten sentences 1. Several types of LED technology are used in monitors, TVs, and mobile devices. Name these technologies and briefly describe the differences. 2. Experts are claiming that 3D printers are going to change a variety of industries. Name at least two of these industries and provide a few examples of the products that will be 3D printed
Please do both! 4. In one to two sentences describe the function of DNA topoisomerases. 5....
Please do both!
4. In one to two sentences describe the function of DNA topoisomerases. 5. Individual amino acids are attached to their corresponding tRNAs: through an ester linkage to the 3-OH in the acceptor stem. through an amide linkage to the anticodon loop. through an amide linkage to the adenine ring at the 3'end of the tRNA. through a phosphoester linkage to the 5' end of the tRNA. None of the above. doooo
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
help!
14. Which of the following factors does effect on DNA fragments separation during electrophoresis a. Size b. Electrical charge c. Nucleotides sequences d. All of the above Match the following with q 15-18 a. A necessary cofactor for DNA polymerization b. To break cell membrane open c. Confirm plant-based sample d. Detection of GMO e. Cell membrane lysis 15. Salt solution and heat 16. Primer of CaMw promoter 17. Primer of Nopalin Synthetase virus terminator 18. Magnesium chloride 19-...
Refer to the picture included in question 7 for this question.
Please explain how gel electrophoresis separates the DNA fragments
that are produced by the Sanger technique. Include the following
elements within your explanation (not necessarily in order of how
you should place them in your answer):
electric current and charge
size of DNA molecules
DNA samples
gel
wells
and buffer solution.
I I III I
b. (4 pt) Describe how active 6mA DNA demethylation occurs.
Answer all questions: C10) 1. You were given to study a linear DNA sample by agarose gel electrophoresis. You were also given a standard DNA size maker. After electrophoresis and staining of DNA, you found several bands in standard DNA size marker (M) and two bands in sample (S) DNA. Size of each standard DNA molecule and their distance from the origin of electrophoresis was provided (see picture below). ) Draw a standard curve using the graph paper provided. Make...
Please do both!
4. In one to two sentences describe the function of DNA topoisomerases. 5. Individual amino acids are attached to their corresponding tRNAs: through an ester linkage to the 3-OH in the acceptor stem. through an amide linkage to the anticodon loop. through an amide linkage to the adenine ring at the 3'end of the tRNA. through a phosphoester linkage to the 5' end of the tRNA. None of the above. doooo
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