1. Explain how Real time PCR has a higher sensitivity and specificity compared to conventional PCR...
1. Explain how Real time PCR has a higher sensitivity and specificity compared to conventional PCR
2. A student has detected a DNA variation (G>A) in exon 1 (coding region of B-globin gene) that does not cause amino acid substitution. But the expression of the B-globin gene was badly affected. Explain why.
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1. Explain how Real time PCR has a higher sensitivity and
specificity compared to conventional PCR...
PLEASE COMPUTER TYPED* OR VERY CLEAR HANDWRITING with details 1. Radioactive (14C) uridine (lots of it)...
PLEASE COMPUTER TYPED* OR VERY CLEAR HANDWRITING with
details
1. Radioactive (14C) uridine (lots of it) is added to a growing culture of bacteria for 10 seconds and then the drug rifamycin is added (rifamycin blocks the initiation of transcription, but not continued elongation of RNA polymerases that have already begun transcribing). At two-minute intervals, 1 ml samples are removed and placed on ice. The bacteria are lysed (broken open) and an enzyme is added that will chew up all...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during Meiosis I? 26. Explain how these inheritance patterns are considered non-Mendelian. Incomplete Dominance . Multiple Alleles • Codominance X-linked Linkage . Pedigrees - Genetic Disorders 27. What is non-disjunction and how does it affect the chromosome distribution during meiosis? 28. What is a karyotype and what does it allow you to do? 29. Fill in the circles and squares to illustrate the following inheritance...
5. Quantitative trait loci(QTL) are: The loci that contribute all of the variation in a continuous...
5. Quantitative trait loci(QTL) are: The loci that contribute all of the variation in a continuous trait The loci that contribute all of the genetic variation in a continuous trait The loci that contribute the broad-sense heritability in a continuous trait The loci that contribute the narrow-sense heritability in a continuous trait 6. We looked at a study where Drosophila flies were selected for phototaxis (either attracted to or repelled by light). This experiment measures how much genetic variation existed...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
explain 1 or 2 molecular details in how epigenetics may allow melanoma cells to outsmart BRAF inhibitors **This is for a genetics course Seope: This science commentary claims that epigeneties pl...
explain 1 or 2 molecular details in how epigenetics may allow
melanoma cells to outsmart BRAF inhibitors
**This is for a genetics course
Seope: This science commentary claims that epigeneties plays a role in cancer cells becoming therapy-resistant. The language used is generalized for the non-scientist and does not include any molecular details, such as methylation of a specific nucleotide in an allele or modification of a specific histone tail amino acid. Your task (individually or in a group of...
explaim the mechanisms amd toxological effects if type 1 diabetes in this article Exposure to arsenic...
explaim the mechanisms amd toxological effects if type 1
diabetes in this article
Exposure to arsenic in drinking water is associated with increased prevalence of diabetes. We previously reported an association of diabetes and urinary concentration of dimethylarsinite (DMAS"), a toxic product of arsenic methylation by arsenic (+ 3 oxidation state) methyltransferase (AS3MT). Here we examine associations between AS3MT polymorphism, arsenic metabolism and diabetes. Fasting blood glucose, oral glucose tolerance and self-reported diagnoses were used to identify diabetic individuals. Inorganic...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
PLEASE COMPUTER TYPED* OR VERY CLEAR HANDWRITING with
details
1. Radioactive (14C) uridine (lots of it) is added to a growing culture of bacteria for 10 seconds and then the drug rifamycin is added (rifamycin blocks the initiation of transcription, but not continued elongation of RNA polymerases that have already begun transcribing). At two-minute intervals, 1 ml samples are removed and placed on ice. The bacteria are lysed (broken open) and an enzyme is added that will chew up all...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during Meiosis I? 26. Explain how these inheritance patterns are considered non-Mendelian. Incomplete Dominance . Multiple Alleles • Codominance X-linked Linkage . Pedigrees - Genetic Disorders 27. What is non-disjunction and how does it affect the chromosome distribution during meiosis? 28. What is a karyotype and what does it allow you to do? 29. Fill in the circles and squares to illustrate the following inheritance...
explain 1 or 2 molecular details in how epigenetics may allow
melanoma cells to outsmart BRAF inhibitors
**This is for a genetics course
Seope: This science commentary claims that epigeneties plays a role in cancer cells becoming therapy-resistant. The language used is generalized for the non-scientist and does not include any molecular details, such as methylation of a specific nucleotide in an allele or modification of a specific histone tail amino acid. Your task (individually or in a group of...
explaim the mechanisms amd toxological effects if type 1
diabetes in this article
Exposure to arsenic in drinking water is associated with increased prevalence of diabetes. We previously reported an association of diabetes and urinary concentration of dimethylarsinite (DMAS"), a toxic product of arsenic methylation by arsenic (+ 3 oxidation state) methyltransferase (AS3MT). Here we examine associations between AS3MT polymorphism, arsenic metabolism and diabetes. Fasting blood glucose, oral glucose tolerance and self-reported diagnoses were used to identify diabetic individuals. Inorganic...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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