Question

what form/s of MAPK does the anti-total map kinase antibody detect, and what form/s of MAPK does the anti-active map kinase antibody detect?

Answer 1

MAPK test

To understand how mitogenic signals are transduced into the trophoblast in preimplantation embryos, the expression of mitogen-activated protien kinase pathway molecules was tested. We used immunocytochemical means and reverse transcriptase - polymerase chain reaction to test whether MAPK pathway molecule gene products exist at the protein and phosphoprotein level in the zygote and the RNA level in the egg and zygote. In addition, all antibodies detected the correct sized major brand in westerns of placental cell lines representing the most prevalent cell type in preimplantation embryos. A majority of mRNA transcripts of MAPK pathway genes were detected in unfertilized eggs, and all were expressed in zygote.  

MAPK Signalling Pathway

The mitogenmitogen- activated protein kinase signalling pathway is activated by a number of extra and intracellular stimuli including cytokines, growth factors, and hormones as well as stressors such as oxidative and ER stress. This pathways plays key role in the regulation of many cellular processes including proliferation, differentiation, the stress response, motility, growth, differentiation, the stress response, motility, growth, differentiation, , survival, and death. Abnormal MAPK signalling may contribute to increased or uncontrolled cell proliferation and or resistance to apoptosis. Dysregulation of controlled MAPK signalling has been shown to be important in the development and progression of many diseases including various types of cancer and neurodegenerative diseases such as alzheimer's disease, parkinson's disease and amyotrophic lateral sclerosis.

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