8. Which of the following is incorrect concerning σ32? a. Translated σ32 associates with DnaJ and...
8. Which of the following is incorrect concerning σ32?
a. Translated σ32 associates with DnaJ and DnaK under normal growth conditions
b. σ32 is transcribed regularly at all temperatures
c. σ32 leads to the transcription of proteins to help combat denaturation
d. σ32 outcompetes σ70 binding to the RNA polymerase at elevated temperatures
e. Access to the RBS for σ32 is blocked by secondary structure at elevated temperatures
6. Which of the following concerning the lac
operon is incorrect?
a. lacI expression is regulated by the levels
of glucose and lactose
b. CAP protein allostery is altered by the concentration
of cAMP
c. CAP cooperatively interacts with RNA polymerase
d. Basal expression of the lac operon occurs when glucose
is present, and lactose is present
e. LacI has greater affinity for its binding site than RNA
polymerase
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8. Which of the following is incorrect concerning
σ32?
a. Translated σ32 associates with DnaJ and...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
The gene machine program shows you what happens when lactose is present in E. coli, and...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
A. Describe in detail the regulation of the prokaryotic lac operon for the following environment: Glucose...
A. Describe in detail the regulation of the prokaryotic lac operon for the following environment: Glucose is present, lactose present, & the repressor protein is mutated such that the allosteric site is non-functional and can no longer bind its substrate. Be sure to specify the presence or absence and location of Lac I, allolactose, cAMP, CAP, and RNA polymerase. B. Indicate whether transcription is occurring at a basal level, a high level, or not at all.
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
26. The lac operon in E. coli consists of genes that code for enzymes necessary for...
26. The lac operon in E. coli consists of genes that code for enzymes necessary for the breakdown of lactose. When lactose is absent, the operon is inactive because a repressor protein binds to a specific site in the lac operon. When lactose is present, lactose molecules bind to the repressor protein, causing the repressor protein to dissociate from the binding site. In the absence of glucose (a preferred energy source for bacteria), the protein CAP binds to a regulatory...
gene regulation of bacteria operon systems. Protected View . Saved to this PG References Mailings Review...
gene regulation of bacteria operon systems. Protected View . Saved to this PG References Mailings Review View Help Tell me what you want to do ernet can contain viruses. Unless you need to edit, it's safe to stay in Protected View Enable Editing 5. What happens if lactose levels are low? Put the following list in order (1-5). RNA polymerase is blocked from transcribing the genes for the lactose metabolizing enzymes When RNA polymerase binds to the promoter, it cannot...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high glucose d) Low lactose, low glucose e) None of the above would have any lac operon expression 2. (2 points) Which of the following is true concerning molecular genetics? a) tRNA carries amino acids into the nucleus in eukaryotic cells b) DNA polymerase moves towards the...
Microbiology study guide questions. Please Help! s. which of the following statements about water activity is not true? a) many fungi can tolerate aw levels of 07-0.8 b) many microorganisms grow...
Microbiology study guide questions. Please Help!
s. which of the following statements about water activity is not true? a) many fungi can tolerate aw levels of 07-0.8 b) many microorganisms grow well at an aw of 0.98 e sear water has an aw of 0.98 e-xerephiles thrive in dry conditions DNA typically becomes disordered below an a of o.90 Which of the following descriptions about ribosome structure is not true? a) 80s is the size of the intact functional ribosome...
Yet, all the cells in your body contain the same genes (and same alleles). The difference...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
1) Which of the following conditions is most likely to cause the lactose operon to be...
1) Which of the following conditions is most likely to cause the lactose operon to be transcribed? A) There is more glucose in the cell than lactose. B) There is glucose but no lactose in the cell. C) The cyclic AMP and lactose levels are both high within the cell. D) The CAMP level is high and the lactose level is low. 2) How does the transcription of structural genes in an inducible operon occur? A) It occurs continuously in...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
26. The lac operon in E. coli consists of genes that code for enzymes necessary for the breakdown of lactose. When lactose is absent, the operon is inactive because a repressor protein binds to a specific site in the lac operon. When lactose is present, lactose molecules bind to the repressor protein, causing the repressor protein to dissociate from the binding site. In the absence of glucose (a preferred energy source for bacteria), the protein CAP binds to a regulatory...
gene regulation of bacteria operon systems. Protected View . Saved to this PG References Mailings Review View Help Tell me what you want to do ernet can contain viruses. Unless you need to edit, it's safe to stay in Protected View Enable Editing 5. What happens if lactose levels are low? Put the following list in order (1-5). RNA polymerase is blocked from transcribing the genes for the lactose metabolizing enzymes When RNA polymerase binds to the promoter, it cannot...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high glucose d) Low lactose, low glucose e) None of the above would have any lac operon expression 2. (2 points) Which of the following is true concerning molecular genetics? a) tRNA carries amino acids into the nucleus in eukaryotic cells b) DNA polymerase moves towards the...
Microbiology study guide questions. Please Help!
s. which of the following statements about water activity is not true? a) many fungi can tolerate aw levels of 07-0.8 b) many microorganisms grow well at an aw of 0.98 e sear water has an aw of 0.98 e-xerephiles thrive in dry conditions DNA typically becomes disordered below an a of o.90 Which of the following descriptions about ribosome structure is not true? a) 80s is the size of the intact functional ribosome...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
1) Which of the following conditions is most likely to cause the lactose operon to be transcribed? A) There is more glucose in the cell than lactose. B) There is glucose but no lactose in the cell. C) The cyclic AMP and lactose levels are both high within the cell. D) The CAMP level is high and the lactose level is low. 2) How does the transcription of structural genes in an inducible operon occur? A) It occurs continuously in...
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