Question

Mg2+ serves the following function(s) in the reaction catalyzed by DNA polymerase.

Multiple answers allowed

A. It stabilizes the pentacoordinate transition state of the phosphodiester bond formation.

B. It precipitates inorganic phosphate (Pi) arising from the reaction.

C. It interacts with the 3'-hydroxyl of the primer and a phosphate in the dNTP.

D. It stabilizes the negative charge on the departing pyrophosphate which is derived from the incoming dNTP.

E. It interacts with the 3' hydroxyl of the incoming dNTP.

Answer 1

Answer is (D)

it stabilizes the negative charge on the departing pyrophosphate which is derived from the incoming dNTP.

Explanation--> in DNA polymerase active site two magnesium ions are present to facilitate the polymerization reaction. one magnesium interacts with the 3' OH of last added nucleotide on the primer. and second magnesium stabilizing the negative charges on the diphosphate leaving group or pyrophosphate which is derived from the incoming dNTP.