Question

9) of the following bacteria which is more resistant to higher temperature a. one which produce capsule b. one which produce flagella c. one which produce spore d. one which produce cell wall 10) Name two genuses of bacteria that undergo spore formation 11) What is a pH in relation to any given media a, sodium concentration in media b. chlorine concentration in media c. hydrogen concentration in media d. none of the above 12) Name two important media ingredients needed for bacterial growth 13) Explain clearly as to why and what acid-fast stain is used for 14) Why are most bacteria incubated at 37 C 15) Based on your laboratory works and experiences, explain what type of test or technique you would apply to distinguish Ecoli from S. aureus

Answer 1

ANSWERS

9. One which produces spore

10. Bacillus (aerobic) and Clostridium (anaerobic)

11. None of the above

12. Nutrient medium and distilled water

13. Acid fast staining is used for identify acid - fast organism such as mycobacterium. Acid - fast stain is a differential stain. Acid - fast organisms contain mycolic acid and large amounts of fatty acids, waxes, and complex lipids. Acid-fast organisms needed special staining technique because their cell wall is resistant to most of the compounds.

14. Most of the bacteria is incubated at 37 °c because it is a optimal temperature. Most of the bacteria grows best in human body temperature that is approximately 37 °c and some bacteria is heat resistant so this is a optimal temperature for the growth of bacterias

15. E M B test can be done where ecoli appears as Greenish, metallic sheen in reflected light with blue black centre and S. aureus appears on the culture as Black and shiny with narrow white margins and surrounded by clear zone.