Question

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The immunoglobulin most often used in immunochemical assays is O IgA Question 6 3 pts A sample of serum contained a concentration of [lgG] in Well 1. You made a series of10-fold dilutions What was the [IgG] in Well 4? Well 1 Well 2 Well 3 Well 4 78.3 mg/ml IgG1:10 dil of Well 1 1:10 dil of Well 2 1:10 dil of Well 3 7.83 ug/mL 0.783 mg/ml O 78.3 mg/mL 0.0783 Hg/mL O 78.3 ug/mL

Answer 1

The Immunoglobulin that is most commonly used in immunochemical assays is IgG. The reason for this is that it is produced in large quantities in the serum in response to the presence of antigens in the body. It accounts for about 80% of all antibodies in the serum and hence, can be easily isolated for laboratory use.

Question 6

Well 1 has 78.3 mg/ml IgG

After 10-fold dilutions, Well 2 has 7.83 mg/ml IgG

After 10-fold dilutions, Well 3 has 0.783 mg/ml IgG

After 10-fold dilutions, Well 4 has 0.0783 mg/ml IgG

In order to convert mg (10^-3) to $\mu$ g (10^-6), we will multiply the number by 1000.

Hence, 0.0783 x 1000 = 78.3.

So, your answer is 78.3 $\mu$ g/ml.