Option D is correct. Chaperones are group of proteins which assist proteins for completion of their folding stages. In general, newly synthesized proteins are linear and may be insoluble and inactive which become active with the help of chaperones by attaining their folding stages during post translational modification. Some bacteria produces inactive, impure and insoluble proteins due to lack of production of chaperones like Hsp70 or Hsp40. In this condition researchers are suggested to use the engineered host system.
Urea can dissolve the protein precipitates but cannot make enzyme active. The activity of enzymes attained only by the proper folding of proteins which is only possible with chaperones. Hence option b is not suitable.
You want to artificially produce an enzyme, protein A. through genetic-engineering. Although can get your "engineered"...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
QUESTION 1 Imagine you want to get rich by genetically engineering tobacco plants to produce acai berry juice. To do so, you insert the genes for each of the enzymes in the hypothetical acai synthesis pathway, the first step of which is (hypothetically) conversion of glucose to fructose. The challenge is that glucose must be maintained at or above a minimum concentration or the plant will die because it can no longer make ATP. For the reaction pathway shown below,...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...