Question

4. Your research adviser hands you a 3-component mixture and tells you to separate them using column chromatography. You already have some lab savvy so you know to test first by tlc to see if you can find a good solvent system for your column. Shown below are your first 2 developed plates and the solvent systems used. Obviously neither system is appropriate, but you now have a good idea of what to try next (you really, really understand this business of polarity). Tell me what is "wrong" with the ones shown and then tell me what solvent system you would try next and why. ethyl acetate:hexanes ethyl acetate:hexanes 3:1 v/v 1:4 v/v

Answer 1

Solution when ever you developed the TLC to separate 3 component or Reaction monitoring in a Lab you must Chouse the combination of solvent je Non polar solvens ratio is more with Combination with polar solvent in given diagrum. Ethyl acetate; hexane (3:1 vlud is wrong combination to developed The becuuse Ethyl acetare is more polar compaired to hexane. and hence polar compounds are not separated in this combination, - while in Ethyl acetate; hexani cl: 4 vlu) is perkect combinanon to separate the 3 componcht. Emyl Acetare hexane Emyl Acetate hexane 3 : 1 CNo separation or Bad Separation) C Better separa non